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Mouse Anti-ECD Recombinant Antibody (1E5) (CBMAB-A4116-LY)

The product is antibody recognizes HSGT1. The antibody 1E5 immunoassay techniques such as: WB, ELISA.
See all ECD antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1E5
Antibody Isotype
IgG1, λ
Application
WB, ELISA

Basic Information

Immunogen
HSGT1 (AAH00721, 1 a.a. ~ 644 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, λ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ECD
Entrez Gene ID
11319
UniProt ID
O95905
Alternative Names
GCR2; HSGT1
Research Area
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).

May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).

Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).

The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).

May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Biological Process
Cell population proliferation Source: Ensembl
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
RNA splicing Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Predominantly is located in the cytoplasm.
PTM
Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.

Mirza, S., Kalluchi, A., Raza, M., Saleem, I., Mohapatra, B., Pal, D., ... & Band, V. (2022). Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical Oncogenesis. Molecular Cancer Research, 20(2), 305-318.

Holding, N., Raza, M., Band, V., Mohapatra, B. C., & Band, H. (2022). Novel Interaction Between ECD and EIF4A1 Indicates ECD Regulates Eukaryotic Translation.

Saleem, I., Mirza, S., Sarkar, A., Raza, M., Mohapatra, B., Mushtaq, I., ... & Band, V. (2021). The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export. Molecular and cellular biology, 41(7), e00103-21.

Erkelenz, S., Stanković, D., Mundorf, J., Bresser, T., Claudius, A. K., Boehm, V., ... & Uhlirova, M. (2021). Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic acids research, 49(3), 1688-1707.

Sarkar, A. (2019). Ecdysoneless, a novel regulator of Ca2+ homeostasis and metabolism.

Sarkar, A., Saleem, I., Mirza, S., Bidasee, K., Rao, R., Band, H., & Band, V. (2018). The novel role of ecdysoneless (ECD) in maintain ER Ca2+ homeostasis. Cancer Research, 78(13_Supplement), 448-448.

Saleem, I., Mirza, S., Sarkar, A., Band, H., & Band, V. (2018). Ecdysoneless, a novel regulator of cell cycle progression and oncogenesis. Cancer Research, 78(13_Supplement), 4454-4454.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell death & disease, 9(5), 1-11.

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For research use only. Not intended for any clinical use.

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