Human Recombinant ECD protein, ECD, GST Tag (V2LY-0526-LY3676)

Name: Human Recombinant ECD protein, ECD, GST Tag
SKU: V2LY-0526-LY3676
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ECD protein, ECD, GST Tag consist of Amino Acid: 1-621 and predicts a molecular mass of 100.6 kDa.

Molecule Mass

100.6 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-621

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ECD

Research Area

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).

May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).

Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).

The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).

May play a role in regulation of pre-mRNA splicing (PubMed:24722212).

Biological Process

Cell population proliferation Source: Ensembl
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
RNA splicing Source: UniProtKB-KW

Cellular Location

Nucleus; Cytoplasm. Predominantly is located in the cytoplasm.

PTM

Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.

Mirza, S., Kalluchi, A., Raza, M., Saleem, I., Mohapatra, B., Pal, D., ... & Band, V. (2022). Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical Oncogenesis. Molecular Cancer Research, 20(2), 305-318.

Holding, N., Raza, M., Band, V., Mohapatra, B. C., & Band, H. (2022). Novel Interaction Between ECD and EIF4A1 Indicates ECD Regulates Eukaryotic Translation.

Saleem, I., Mirza, S., Sarkar, A., Raza, M., Mohapatra, B., Mushtaq, I., ... & Band, V. (2021). The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export. Molecular and cellular biology, 41(7), e00103-21.

Erkelenz, S., Stanković, D., Mundorf, J., Bresser, T., Claudius, A. K., Boehm, V., ... & Uhlirova, M. (2021). Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic acids research, 49(3), 1688-1707.

Sarkar, A. (2019). Ecdysoneless, a novel regulator of Ca2+ homeostasis and metabolism.

Sarkar, A., Saleem, I., Mirza, S., Bidasee, K., Rao, R., Band, H., & Band, V. (2018). The novel role of ecdysoneless (ECD) in maintain ER Ca2+ homeostasis. Cancer Research, 78(13_Supplement), 448-448.

Saleem, I., Mirza, S., Sarkar, A., Band, H., & Band, V. (2018). Ecdysoneless, a novel regulator of cell cycle progression and oncogenesis. Cancer Research, 78(13_Supplement), 4454-4454.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell death & disease, 9(5), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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