Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406) (V2LY-0425-LY1335)

Name: Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406)
SKU: V2LY-0425-LY1335
Rating: 5 (1 reviews)

See all ECH1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406) (V2LY-0425-LY1335-1) in WB

(A) and (B) on the left side: representative immunoblots of three independent biological replicates are shown. On the right side: the respective quantification of the immunoblots as described in Materials and Methods. Error bars=mean±SD of three independent experiments. Statistical analysis: 1-way ANOVA with Dunnett's multiple comparison test; (**** = p≤0.0001). ...View More

Schmitt, L., Krings, K. S., Wolsing, A., Buque, X., Zimmermann, M., Flores-Romero, H., ... & Wesselborg, S. (2024). Targeting mitochondrial metabolism by the mitotoxin bromoxib in leukemia and lymphoma cells. Cell Communication and Signaling, 22(1), 541.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0406

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 47-65 near the N-terminus of human ECH1.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

enoyl Coenzyme A hydratase 1, peroxisomal

Entrez Gene ID

Human	1891
Mouse	51798
Rat	64526

UniProt ID

Human	Q13011
Mouse	O35459
Rat	Q62651

Research Area

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.

Biological Process

Fatty acid beta-oxidation Source: UniProtKB-UniPathway

Cellular Location

Mitochondrion; Peroxisome

Dao, C. K., Kenaston, A., Hirasaka, K., Kohno, S., Riley, C., Fang, G., ... & Mills, E. M. (2019). Identification of a fatty acid-sensitive interaction between mitochondrial uncoupling protein 3 and enoyl-CoA hydratase 1 in skeletal muscle. bioRxiv, 821637.

Sharma, N. K., Chuang Key, C. C., Civelek, M., Wabitsch, M., Comeau, M. E., Langefeld, C. D., ... & Das, S. K. (2019). Genetic regulation of Enoyl-CoA hydratase domain-containing 3 in adipose tissue determines insulin sensitivity in African Americans and Europeans. Diabetes, 68(7), 1508-1522.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406) (CAT#: CBMAB-E0718-FY)

Related Products

Mouse Anti-ECH1 Recombinant Antibody (5G8) (CAT#: V2LY-0425-LY1334)

Mouse Anti-ECH1 Recombinant Antibody (CBFYE-0406) (CAT#: CBMAB-E0718-FY)

Mouse Anti-ECH1 Monoclonal Antibody (5G8) (CAT#: CBMAB-1122-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry