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Mouse Anti-ECR Recombinant Antibody (9B9) (CBMAB-0323-CN)

This product is a mouse antibody that recognizes ECR of Manduca sexta. The antibody 9B9 can be used for immunoassay techniques such as: ELISA.
See all ECR antibodies

Summary

Host Animal
Mouse
Specificity
Manduca sexta, Manduca, Silkworm, House cricket
Clone
9B9
Antibody Isotype
IgG2b
Application
ELISA

Basic Information

Specificity
Manduca sexta, Manduca, Silkworm, House cricket
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EcR
Introduction
The ecdysone receptor is a nuclear receptor found in arthropods, where it controls development and contributes to other processes such as reproduction. The receptor is a non-covalent heterodimer of two proteins, the EcR protein and ultraspiracle protein (USP). This protein binds to and is activated by ecdysteroids. Insect ecdysone receptors are currently better characterized than those from other arthropods, and mimics of ecdysteroids are used commercially as caterpillar-selective insecticides.
UniProt ID
Alternative Names
20-hydroxy-ecdysone receptor; 20E receptor; EcRH
Biological Process
Ecdysone receptor-mediated signaling pathway Source: InterPro
Cellular Location
Nucleus

Maharaj, S., Ekoka, E., Erlank, E., Nardini, L., Reader, J., Birkholtz, L. M., & Koekemoer, L. L. (2022). The ecdysone receptor regulates several key physiological factors in Anopheles funestus. Malaria journal, 21(1), 1-13.

Wu, J. J., Mu, L. L., Kang, W. N., Ze, L. J., Shen, C. H., Jin, L., ... & Li, G. Q. (2021). RNA interference targeting ecdysone receptor blocks the larval–pupal transition in Henosepilachna vigintioctopunctata. Insect science, 28(2), 419-429.

He, Y. Z., Aksoy, E., Ding, Y., & Raikhel, A. S. (2021). Hormone-dependent activation and repression of microRNAs by the ecdysone receptor in the dengue vector mosquito Aedes aegypti. Proceedings of the National Academy of Sciences, 118(26), e2102417118.

Abdullah‐Zawawi, M. R., Afiqah‐Aleng, N., Ikhwanuddin, M., Sung, Y. Y., Tola, S., Fazhan, H., & Waiho, K. (2021). Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture. Reviews in Aquaculture, 13(4), 1938-1957.

Yogindran, S., & Rajam, M. V. (2021). Host-derived artificial miRNA-mediated silencing of ecdysone receptor gene provides enhanced resistance to Helicoverpa armigera in tomato. Genomics, 113(1), 736-747.

Petruccelli, E., Lark, A., Mrkvicka, J. A., & Kitamoto, T. (2020). Significance of DopEcR, a G-protein coupled dopamine/ecdysteroid receptor, in physiological and behavioral response to stressors. Journal of neurogenetics, 34(1), 55-68.

Li, X., Ren, X., Liu, Y., Smagghe, G., Liang, P., & Gao, X. (2020). MiR-189942 regulates fufenozide susceptibility by modulating ecdysone receptor isoform B in Plutella xylostella (L.). Pesticide biochemistry and physiology, 163, 235-240.

Mellor, C. L., Tollefsen, K. E., LaLone, C., Cronin, M. T., & Firman, J. W. (2020). In silico identification of chemicals capable of binding to the ecdysone receptor. Environmental toxicology and chemistry, 39(7), 1438-1450.

Chan, Y. H., Chu, K. H., & Chan, K. M. (2019). Ecdysteroid-mimicking compounds act as both agonists and antagonists to the crustacean ecdysone receptor. Chemosphere, 237, 124551.

Lenaerts, C., Marchal, E., Peeters, P., & Vanden Broeck, J. (2019). The ecdysone receptor complex is essential for the reproductive success in the female desert locust, Schistocerca gregaria. Scientific reports, 9(1), 1-12.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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