Mouse Anti-ECRB1 Monoclonal Antibody (10F1) (V2LY-0425-LY1354)

Name: Mouse Anti-ECRB1 Monoclonal Antibody (10F1)
SKU: V2LY-0425-LY1354
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ECR Monoclonal Antibody (10F1) (V2LY-0425-LY1354-1) in IF

Larval wings immunostained with EcR (Red) and pH3 (Green) antibodies at two stages of Wr stage-40% and 90% development, zoomed in to show the developing dorsal Cu1 eyespot centers. Scale bars, 20μm. ...View More

Bhardwaj, S., Prudic, K. L., Bear, A., Dasgupta, M., Wasik, B. R., Tong, X., ... & Monteiro, A. (2018). Sex differences in 20-hydroxyecdysone hormone levels control sexual dimorphism in Bicyclus anynana wing patterns. Molecular Biology and Evolution, 35(2), 465-472.

Mouse Anti-ECR Monoclonal Antibody (10F1) (V2LY-0425-LY1354-2) in IF

Earlier during the 5th instar, however, EcR is expressed in both forewing (left) and hindwing (right) eyespot centers on the ventral surface. Arrows point to the Cu1 eyespot. ...View More

Monteiro, A., Tong, X., Bear, A., Liew, S. F., Bhardwaj, S., Wasik, B. R., ... & Prudic, K. L. (2015). Differential expression of ecdysone receptor leads to variation in phenotypic plasticity across serial homologs. PLoS Genetics, 11(9), e1005529.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

10F1

Application

IF, IHC, WB

Immunogen

Amino acids 33-454 of EcR-B1.

Host Species

Mouse

Specificity

Bombyx mori, Common fruit fly, Locusta migratoria, Manduca, Rhodnius prolixus

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	0.2-0.5 µg/ml
IF(ICC)	2-5 µg/ml
IHC	2-5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ecdysone receptor (EcR) B1-isoform

UniProt ID

P49883

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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