Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425)

Name: Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031)
SKU: V2LY-0425-LY1425
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425-1) in IF

Double immunofluorescence staining for TLR9 and early endosomal antigen 1 (EEA1) as a marker of early endosomes. Sections of cervical DRG ipsilateral (DRGC7i) to sham operation (a-c) and CSNT, (d-f) were immunostained with rabbit polyclonal anti-TLR9 (a,d) and mouse monoclonal anti-EEA1 (b,e) antibodies. TLR9 and EEA1 were visualized with TRITC-conjugated donkey anti-rabbit and FITC-conjugated donkey anti-mouse secondary antibodies, respectively. Cell nuclei were detected with Hoechst 33342. More such punctate double immunostaining was found in the neurons of DRGC7i from CSNT-operated rat. Scale bars = 10 µm. ...View More

Dubový, P., Hradilová-Svíženská, I., Brázda, V., & Joukal, M. (2021). Toll-like receptor 9-mediated neuronal innate immune reaction is associated with initiating a pro-regenerative state in neurons of the dorsal root ganglia non-associated with sciatic nerve lesion. International Journal of Molecular Sciences, 22(14), 7446.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425-2) in WB

Polysome profile of U251 cells untreated (NT) or puromycin treated (Puro), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. ...View More

Herviou, P., Le Bras, M., Dumas, L., Hieblot, C., Gilhodes, J., Cioci, G., ... & Millevoi, S. (2020). hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nature communications, 11(1), 2661.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425-3) in IF

RIPosomes co-localization with cellular compartments and signalling platforms.
Indirect immunofluorescence micrographs of HeLa EGFP-RIPK2 cells infected with S. flexneri M90T or BS176 for 2 h. TIFA-Flag and Flag-TRAF6 were transfected 16 h before doxycycline induction. EGFP-RIPK2 (green), immunostaining with anti-Flag, anti-AIF, anti-SMN, anti-Gemin3, anti-LC3β, or anti-EEA1 antibodies (shown in red) together with DNA staining (blue) are shown. Scale bar = 10 μm. ...View More

Ellwanger, K., Briese, S., Arnold, C., Kienes, I., Heim, V., Nachbur, U., & Kufer, T. A. (2019). XIAP controls RIPK2 signaling by preventing its deposition in speck-like structures. Life Science Alliance, 2(4).

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425-4) in IF

Trafficking of CARDS toxin through early and late endosomes and the Golgi complex to reach the ER. HeLa cells were treated with CARDS toxin, shifted to 37°C, incubated for 15 min to 8 h, and fixed and processed for coimmunofluorescence analysis (co-IFA). Early endosomes were labeled using mouse anti-EEA1 monoclonal antibody (1:100), late endosomes using anti-Rab9 antibody (1:100), Golgi complex using anti-GM130 antibody (1:1,000), and ER using anticalnexin antibody (1:100). The primary antibodies were counterstained using secondary antibody conjugated with Alexa Fluor 488 (green; 1:1,000). CARDS toxin was labeled with rabbit anti-CARDS toxin polyclonal antibody (1:1,000) and stained with Alexa Fluor 555-conjugated secondary antibody (red; 1:1,000). Nuclei were stained with DAPI (blue). Images were collected sequentially from different channels by immunofluorescence microscopy. The merged images show colocalization of CARDS toxin with different organelles. The boxed insets in the merged panels show magnified views of both colocalized and distinct patterns of CARDS toxin and specific organelle staining. (A) EEA1 and CARDS toxin at 1 h. (B) Rab9 and CARDS toxin at 2 h. (C) GM130 and CARDS toxin at 4 h. (D) Cells pretreated with BFA (10 μg/ml), incubated with CARDS toxin for 4 h, and immunostained for GM130 and CARDS toxin. (E) Calnexin and CARDS toxin at 8 h. ...View More

Ramasamy, K., Balasubramanian, S., Manickam, K., Pandranki, L., Taylor, A. B., Hart, P. J., ... & Kannan, T. R. (2018). Mycoplasma pneumoniae community-acquired respiratory distress syndrome toxin uses a novel KELED sequence for retrograde transport and subsequent cytotoxicity. MBio, 9(1), 10-1128.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0031) (V2LY-0425-LY1425-5) in IF

HeLa cells were transfected with plasmid encoding GFP-clavesin 1 and subsequently processed by indirect immunofluorescence with antibody against EEA1 (red). The lower panels are from the area indicated by the white box in the merge image. Bars, 10 and 2 μm for the low and high power images, respectively. ...View More

Katoh, Y., Ritter, B., Gaffry, T., Blondeau, F., Höning, S., & McPherson, P. S. (2009). The clavesin family, neuron-specific lipid-and clathrin-binding Sec14 proteins regulating lysosomal morphology. Journal of Biological Chemistry, 284(40), 27646-27654.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0031

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 82-1411 of human EEA1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Early Endosome Antigen 1

Entrez Gene ID

Human	8411
Mouse	216238
Rat	314764

UniProt ID

Human	Q15075
Mouse	Q8BL66
Rat	F1LUA1

Research Area

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Biological Process

Early endosome to late endosome transport Source: UniProtKB
Endocytosis Source: UniProtKB
Synaptic vesicle to endosome fusion Source: UniProtKB
Vesicle fusion Source: UniProtKB
Viral RNA genome replication Source: ParkinsonsUK-UCL

Cellular Location

Early endosome membrane; Cytoplasm

McGowan, S. E., & McCoy, D. M. (2021). Neuropilin-1 directs PDGFRα-entry into lung fibroblasts and signaling from very early endosomes. American Journal of Physiology-Lung Cellular and Molecular Physiology, 320(2), L179-L192.

Kamentseva, R., Kosheverova, V., Kharchenko, M., Zlobina, M., Salova, A., Belyaeva, T., ... & Kornilova, E. (2020). Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway. PLoS One, 15(5), e0232532.

Baroja-Mazo, A., Compan, V., Martín-Sánchez, F., Tapia-Abellán, A., Couillin, I., & Pelegrín, P. (2019). Early endosome autoantigen 1 regulates IL-1β release upon caspase-1 activation independently of gasdermin D membrane permeabilization. Scientific reports, 9(1), 1-10.

Posiri, P., Thongsuksangcharoen, S., Chaysri, N., Panyim, S., & Ongvarrasopone, C. (2019). PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon. Fish & shellfish immunology, 95, 449-455.

Miller, C. M., Wan, W. B., Seth, P. P., & Harris, E. N. (2018). Endosomal escape of antisense oligonucleotides internalized by stabilin receptors is regulated by Rab5C and EEA1 during endosomal maturation. Nucleic acid therapeutics, 28(2), 86-96.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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