Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427)

Name: Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450)
SKU: V2LY-0425-LY1427
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427-1) in IF

Representative immunofluorescent photomicrographs of TLR4 and CaM localization in the LPS-stimulated neonatal mice
cardiomyocytes labeled with PDI (endoplasmic reticulum marker), EEA1 (early endosome marker), and Rab7 (late endosome marker). Blue indicates cellular nuclei stained with DAPI. Representative colocalization is indicated by white arrows (n = 6 images from 3 biological independent experiments). Source data are provided as a Source Data file. ...View More

Tang, N., Tian, W., Ma, G. Y., Xiao, X., Zhou, L., Li, Z. Z., ... & Cao, W. (2022). TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca2+ leakage. Nature Communications, 13(1), 7455.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427-2) in IF

Confocal laser scanning microscopy analysis of 293TPSCA cells treated with scFv(AM1)-P-BAP or scFv(MR1.1)-P-BAP hybrid polyplexes containing Cy3-labelled plasmid DNA. To visualize the route of internalisation, cells were additionally stained with wheat germ agglutinin or early endosomal marker EEA1. Arrows depict Cy3-labelled pDNA or early endosomes. ...View More

Jugel, W., Tietze, S., Daeg, J., Appelhans, D., Broghammer, F., Aigner, A., ... & Temme, A. (2022). Targeted Transposition of Minicircle DNA Using Single-Chain Antibody Conjugated Cyclodextrin-Modified Poly (Propylene Imine) Nanocarriers. Cancers, 14(8), 1925.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427-3) in IF

HEK-293 cells transfected with hAQP5-ΔCT were analyzed for the cellular localization of the mutants and EEA1 by immunofluorescence. ...View More

Muroi, S. I., & Isohama, Y. (2021). C-terminal domain of Aquaporin-5 is required to pass its protein quality control and ensure its trafficking to plasma membrane. International journal of molecular sciences, 22(24), 13461.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427-4) in IF

Confocal microscopic images of epithelial cells incubated in either medium alone (top) or infected with C. albicans (bottom) for 90 min. Red spots indicate the regions where the indicated proteins associate with EGFR. Results are representative of three independent experiments. Scale bar 25 µm. ...View More

Phan, Q. T., Lin, J., Solis, N. V., Eng, M., Swidergall, M., Wang, F., ... & Filler, S. G. (2021). The globular C1q receptor is required for epidermal growth factor receptor signaling during Candida albicans infection. MBio, 12(6), e02716-21.

Mouse Anti-EEA1 Recombinant Antibody (CBFYE-0450) (V2LY-0425-LY1427-5) in IF

(a,b) Murine ubiquitin-deficient and control BM-DC were stimulated with nicotine (10−7 mol/L) for 12~16 h. Then, the cells were replenished with K48R-Ub or WT-Ub (30 × 10−6 mol/L) and further incubated with ovalbumin (50 μg/mL) for 5~6 h. The endosomal relocation of p97 (a) from the endoplasmic reticulum (b) was determined by confocal microscope with EEA1, calnexin, and p97 antibody staining. EEA1 (a), calnexin (b) were stained green; p97 was stained red; nuclei were counterstained with DAPI (blue). ...View More

Hu, C. F., Liao, X. Y., Xu, D. D., Ruan, Y. B., & Gao, F. G. (2021). K48-linked ubiquitination contributes to nicotine-augmented bone marrow-derived dendritic-cell-mediated adaptive immunity. Vaccines, 9(3), 278.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0450

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 2-27 at the N-terminus of human EEA1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Early Endosome Antigen 1

Entrez Gene ID

Human	8411
Mouse	216238
Rat	314764

UniProt ID

Human	Q15075
Mouse	Q8BL66
Rat	F1LUA1

Research Area

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Biological Process

Early endosome to late endosome transport Source: UniProtKB
Endocytosis Source: UniProtKB
Synaptic vesicle to endosome fusion Source: UniProtKB
Vesicle fusion Source: UniProtKB
Viral RNA genome replication Source: ParkinsonsUK-UCL

Cellular Location

Early endosome membrane; Cytoplasm

McGowan, S. E., & McCoy, D. M. (2021). Neuropilin-1 directs PDGFRα-entry into lung fibroblasts and signaling from very early endosomes. American Journal of Physiology-Lung Cellular and Molecular Physiology, 320(2), L179-L192.

Kamentseva, R., Kosheverova, V., Kharchenko, M., Zlobina, M., Salova, A., Belyaeva, T., ... & Kornilova, E. (2020). Functional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway. PLoS One, 15(5), e0232532.

Baroja-Mazo, A., Compan, V., Martín-Sánchez, F., Tapia-Abellán, A., Couillin, I., & Pelegrín, P. (2019). Early endosome autoantigen 1 regulates IL-1β release upon caspase-1 activation independently of gasdermin D membrane permeabilization. Scientific reports, 9(1), 1-10.

Posiri, P., Thongsuksangcharoen, S., Chaysri, N., Panyim, S., & Ongvarrasopone, C. (2019). PmEEA1, the early endosomal protein is employed by YHV for successful infection in Penaeus monodon. Fish & shellfish immunology, 95, 449-455.

Miller, C. M., Wan, W. B., Seth, P. P., & Harris, E. N. (2018). Endosomal escape of antisense oligonucleotides internalized by stabilin receptors is regulated by Rab5C and EEA1 during endosomal maturation. Nucleic acid therapeutics, 28(2), 86-96.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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