Mouse Anti-EEF1A1 Recombinant Antibody (23C11) (CBMAB-E0795-FY)

Name: Mouse Anti-EEF1A1 Recombinant Antibody (23C11)
SKU: CBMAB-E0795-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

23C11

Application

WB, IF

Immunogen

Recombinant human EEF1A1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-462

More Infomation

Target

Full Name

Eukaryotic Translation Elongation Factor 1 Alpha 1

Introduction

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.

Entrez Gene ID

1915

UniProt ID

P68104

Alternative Names

Eukaryotic Translation Elongation Factor 1 Alpha 1; Leukocyte Receptor Cluster (LRC) Member 7; Leukocyte Receptor Cluster Member 7; Eukaryotic Elongation Factor 1 A-1; Elongation Factor Tu; EF-1-Alpha-1; EEF1A-1; EEF1A; LENG7; EF-Tu; EF1A; Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14; Glucocorticoid Receptor AF-1 Specific Elongation Factor; Translation Elongation Factor 1 Alpha 1-Like 14

Research Area

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976).

(Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection.

Biological Process

Cellular response to epidermal growth factor stimulus Source: UniProtKB
Regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
Regulation of D-erythro-sphingosine kinase activity Source: UniProtKB
Translation Source: GO_Central
Translational elongation Source: GO_Central

Cellular Location

Nucleus; Nucleolus; Cell membrane; Cytoplasm. Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).

PTM

ISGylated.
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2 (PubMed:26497934).
Trimethylated at Lys-79 by EEF1AKMT1 (PubMed:26545399). Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation (PubMed:28108655). Trimethylated at Lys-318 by EEF1AKMT2 (PubMed:25144183). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (PubMed:28520920). Trimethylated at Gly-2 by METTL13 (PubMed:30143613). Mono- and dimethylated at Lys-55 by METTL13; dimethylated form is predominant (PubMed:30143613, PubMed:30612740).

Song, H., Wang, J., Wang, X., Yuan, B., Li, D., Hu, A., ... & Tong, Q. (2022). HNF4A-AS1-encoded small peptide promotes self-renewal and aggressiveness of neuroblastoma stem cells via eEF1A1-repressed SMAD4 transactivation. Oncogene, 41(17), 2505-2519.

Wefers, Z., Alecki, C., Huang, R., Jacob-Tomas, S., & Vera, M. (2022). Analysis of the Expression and Subcellular Distribution of eEF1A1 and eEF1A2 mRNAs during Neurodevelopment. Cells, 11(12), 1877.

Mills, A., & Gago, F. (2021). On the need to tell apart fraternal twins eEF1A1 and eEF1A2, and their respective outfits. International Journal of Molecular Sciences, 22(13), 6973.

Shi, N., Zhang, S., Guo, Y., Yu, X., Zhao, W., Zhang, M., ... & Duan, M. (2021). CircRNA_0050463 promotes influenza A virus replication by sponging miR-33b-5p to regulate EEF1A1. Veterinary Microbiology, 254, 108995.

Wu, A., Tang, J., Guo, Z., Dai, Y., Nie, J., Hu, W., ... & Zhou, G. (2021). Long non-coding RNA CRYBG3 promotes lung cancer metastasis via activating the eEF1A1/MDM2/MTBP axis. International journal of molecular sciences, 22(6), 3211.

Duman, M., Vaquié, A., Nocera, G., Heller, M., Stumpe, M., Siva Sankar, D., ... & Jacob, C. (2020). EEF1A1 deacetylation enables transcriptional activation of remyelination. Nature communications, 11(1), 1-16.

Xu, S., Wu, X., Zhang, X., Chen, C., Chen, H., & She, F. (2020). CagA orchestrates eEF1A1 and PKCδ to induce interleukin-6 expression in Helicobacter pylori-infected gastric epithelial cells. Gut pathogens, 12(1), 1-11.

Li, X., Chen, N., Zhou, L., Wang, C., Wen, X., Jia, L., ... & Li, W. (2019). Genome-wide target interactome profiling reveals a novel EEF1A1 epigenetic pathway for oncogenic lncRNA MALAT1 in breast cancer. American journal of cancer research, 9(4), 714.

Lin, C. Y., Beattie, A., Baradaran, B., Dray, E., & Duijf, P. H. (2018). Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress. Scientific reports, 8(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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