Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (0514) (V2LY-0425-LY1473)

Name: Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (0514)
SKU: V2LY-0425-LY1473
Rating: 5 (1 reviews)

See all EEF2K antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

0514

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids Ser 366 Ser 366 of human Eef2k.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EEF2K

Entrez Gene ID

Human	29904
Mouse	13631
Rat	25435

UniProt ID

Human	O00418
Mouse	O08796
Rat	P70531

Research Area

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Biological Process

Cellular response to anoxia Source: Ensembl
Cellular response to brain-derived neurotrophic factor stimulus Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to cAMP Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Positive regulation of dendritic spine morphogenesis Source: Ensembl
Positive regulation of endocytosis Source: Ensembl
Positive regulation of synapse assembly Source: Ensembl
Protein autophosphorylation Source: UniProtKB
Regulation of protein autophosphorylation Source: Ensembl
Response to ischemia Source: Ensembl
Response to prolactin Source: Ensembl
Translational elongation Source: ProtInc

Cellular Location

Cytosol; Cytoplasm; Dendritic spine; Postsynaptic density

PTM

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

Beretta, S., Gritti, L., Ponzoni, L., Scalmani, P., Mantegazza, M., Sala, M., ... & Sala, C. (2022). Rescuing epileptic and behavioral alterations in a Dravet syndrome mouse model by inhibiting eukaryotic elongation factor 2 kinase (eEF2K). Molecular autism, 13(1), 1-22.

Onder, F. C., Siyah, P., Durdagi, S., Ay, M., & Ozpolat, B. (2022). Novel Etodolac Derivatives as Eukaryotic Elongation Factor 2 Kinase (eEF2K) Inhibitors for Targeted Cancer Therapy. RSC Medicinal Chemistry.

Ma, T. (2021). Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal plasticity, cognition, and Alzheimer disease. Journal of Neurochemistry.

Zhu, S., Liao, M., Tan, H., Zhu, L., Chen, Y., He, G., & Liu, B. (2021). Inhibiting Eukaryotic Elongation Factor 2 Kinase: An Update on Pharmacological Small-Molecule Compounds in Cancer. Journal of Medicinal Chemistry, 64(13), 8870-8883.

Zhang, B., Zou, J., Zhang, Q., Wang, Z., Wang, N., He, S., ... & Naman, C. B. (2021). Progress in the development of eukaryotic elongation factor 2 kinase (eEF2K) natural product and synthetic small molecule inhibitors for cancer chemotherapy. International Journal of Molecular Sciences, 22(5), 2408.

Karakas, D., & Ozpolat, B. (2020). Eukaryotic elongation factor-2 kinase (eEF2K) signaling in tumor and microenvironment as a novel molecular target. Journal of Molecular Medicine, 98(6), 775-787.

Zhou, Y., Li, Y., Xu, S., Lu, J., Zhu, Z., Chen, S., ... & Shen, K. (2020). Eukaryotic elongation factor 2 kinase promotes angiogenesis in hepatocellular carcinoma via PI3K/Akt and STAT3. International journal of cancer, 146(5), 1383-1395.

Ng, T. H., Sham, K. W., Xie, C. M., Ng, S. S., To, K. F., Tong, J. H., ... & Cheng, C. H. (2019). Eukaryotic elongation factor-2 kinase expression is an independent prognostic factor in colorectal cancer. BMC cancer, 19(1), 1-9.

Jan, A., Jansonius, B., Delaidelli, A., An, Y. A., Ferreira, N., Smits, L. M., ... & Sorensen, P. H. (2018). Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity. Acta neuropathologica communications, 6(1), 1-17.

Pan, Z., Chen, Y., Liu, J., Jiang, Q., Yang, S., Guo, L., & He, G. (2018). Design, synthesis, and biological evaluation of polo-like kinase 1/eukaryotic elongation factor 2 kinase (PLK1/EEF2K) dual inhibitors for regulating breast cancer cells apoptosis and autophagy. European journal of medicinal chemistry, 144, 517-528.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (0514)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (EG1034) (CAT#: V2LY-0425-LY1474)

Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (EG1035) (CAT#: V2LY-0425-LY1475)

Mouse Anti-EEF2K Recombinant Antibody (10E8) (CAT#: CBMAB-0511-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry