Human Recombinant EEF2K, Active protein, GST Tag (V2LY-0526-LY3692)

Name: Human Recombinant EEF2K, Active protein, GST Tag
SKU: V2LY-0526-LY3692
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant EEF2K, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 125 kDa.

Molecule Mass

125 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

EEF2K

Research Area

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Biological Process

Cellular response to anoxia Source: Ensembl
Cellular response to brain-derived neurotrophic factor stimulus Source: Ensembl
Cellular response to calcium ion Source: Ensembl
Cellular response to cAMP Source: Ensembl
Cellular response to insulin stimulus Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Positive regulation of dendritic spine morphogenesis Source: Ensembl
Positive regulation of endocytosis Source: Ensembl
Positive regulation of synapse assembly Source: Ensembl
Protein autophosphorylation Source: UniProtKB
Regulation of protein autophosphorylation Source: Ensembl
Response to ischemia Source: Ensembl
Response to prolactin Source: Ensembl
Translational elongation Source: ProtInc

Cellular Location

Cytosol; Cytoplasm; Dendritic spine; Postsynaptic density

PTM

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

Beretta, S., Gritti, L., Ponzoni, L., Scalmani, P., Mantegazza, M., Sala, M., ... & Sala, C. (2022). Rescuing epileptic and behavioral alterations in a Dravet syndrome mouse model by inhibiting eukaryotic elongation factor 2 kinase (eEF2K). Molecular autism, 13(1), 1-22.

Onder, F. C., Siyah, P., Durdagi, S., Ay, M., & Ozpolat, B. (2022). Novel Etodolac Derivatives as Eukaryotic Elongation Factor 2 Kinase (eEF2K) Inhibitors for Targeted Cancer Therapy. RSC Medicinal Chemistry.

Ma, T. (2021). Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal plasticity, cognition, and Alzheimer disease. Journal of Neurochemistry.

Zhu, S., Liao, M., Tan, H., Zhu, L., Chen, Y., He, G., & Liu, B. (2021). Inhibiting Eukaryotic Elongation Factor 2 Kinase: An Update on Pharmacological Small-Molecule Compounds in Cancer. Journal of Medicinal Chemistry, 64(13), 8870-8883.

Zhang, B., Zou, J., Zhang, Q., Wang, Z., Wang, N., He, S., ... & Naman, C. B. (2021). Progress in the development of eukaryotic elongation factor 2 kinase (eEF2K) natural product and synthetic small molecule inhibitors for cancer chemotherapy. International Journal of Molecular Sciences, 22(5), 2408.

Karakas, D., & Ozpolat, B. (2020). Eukaryotic elongation factor-2 kinase (eEF2K) signaling in tumor and microenvironment as a novel molecular target. Journal of Molecular Medicine, 98(6), 775-787.

Zhou, Y., Li, Y., Xu, S., Lu, J., Zhu, Z., Chen, S., ... & Shen, K. (2020). Eukaryotic elongation factor 2 kinase promotes angiogenesis in hepatocellular carcinoma via PI3K/Akt and STAT3. International journal of cancer, 146(5), 1383-1395.

Ng, T. H., Sham, K. W., Xie, C. M., Ng, S. S., To, K. F., Tong, J. H., ... & Cheng, C. H. (2019). Eukaryotic elongation factor-2 kinase expression is an independent prognostic factor in colorectal cancer. BMC cancer, 19(1), 1-9.

Jan, A., Jansonius, B., Delaidelli, A., An, Y. A., Ferreira, N., Smits, L. M., ... & Sorensen, P. H. (2018). Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity. Acta neuropathologica communications, 6(1), 1-17.

Pan, Z., Chen, Y., Liu, J., Jiang, Q., Yang, S., Guo, L., & He, G. (2018). Design, synthesis, and biological evaluation of polo-like kinase 1/eukaryotic elongation factor 2 kinase (PLK1/EEF2K) dual inhibitors for regulating breast cancer cells apoptosis and autophagy. European journal of medicinal chemistry, 144, 517-528.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-EEF2K (Phospho-Ser366) Recombinant Antibody (EG1035) (CAT#: V2LY-0425-LY1475)

Isotype control

For research use only. Not intended for any clinical use.

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