Mouse Anti-EFNA5 Recombinant Antibody (1A2) (V2LY-0425-LY1520)

Name: Mouse Anti-EFNA5 Recombinant Antibody (1A2)
SKU: V2LY-0425-LY1520
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A2

Application

WB, ELISA

Immunogen

Amino acid 114-203 of human ephrin-A5.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ephrin A5

Entrez Gene ID

Human	1946
Mouse	13640
Rat	116683

UniProt ID

Human	P52803
Mouse	O08543
Rat	P97605

Research Area

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

Biological Process

Axon guidance Source: GO_Central
Ephrin receptor signaling pathway Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: MGI
Nervous system development Source: ProtInc
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cell-cell adhesion Source: UniProtKB
Regulation of cell morphogenesis Source: MGI
Regulation of focal adhesion assembly Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Regulation of microtubule cytoskeleton organization Source: UniProtKB

Cellular Location

Cell membrane; Caveola. Compartmentalized in discrete caveolae-like membrane microdomains.

Xie, J., Xing, S., Shen, B. Y., Chen, H. T., Sun, B., Wang, Z. T., ... & Lu, X. X. (2021). PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth via regulating EFNA5. Human Cell, 34(2), 550-563.

Iguchi, T., Oka, Y., Yasumura, M., Omi, M., Kuroda, K., Yagi, H., ... & Sato, M. (2021). Mutually repulsive EphA7–EfnA5 organize region-to-region corticopontine projection by inhibiting collateral extension. Journal of Neuroscience, 41(22), 4795-4808.

Li, S., Hou, X., Wu, C., Han, L., Li, Q., Wang, J., & Luo, S. (2020). MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5. Biomedicine & Pharmacotherapy, 125, 109889.

Millner, T. O., Ricci, B., Zhang, X., Pomella, N., & Marino, S. (2019). Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma. Neuro-Oncology, 21(Suppl 4), iv1.

Pienkowski, V. M., Kucharczyk, M., Młynek, M., Szczałuba, K., Rydzanicz, M., Poszewiecka, B., ... & Płoski, R. (2019). Mapping of breakpoints in balanced chromosomal translocations by shallow whole-genome sequencing points to EFNA5, BAHD1 and PPP2R5E as novel candidates for genes causing human Mendelian disorders. Journal of Medical Genetics, 56(2), 104-112.

Jukonen, J. (2019). EfnA5 is a Non-Canonical Eph-Receptor Ligand and an Unfavorable Factor in High-Grade Serous Ovarian Cancer.

Zhou, Y., & Shiels, A. (2018). Epha2 and Efna5 participate in lens cell pattern-formation. Differentiation, 102, 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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