Rat Recombinant EFNA5 protein, hFc Tag (V2LY-0526-LY9359)

Name: Rat Recombinant EFNA5 protein, hFc Tag
SKU: V2LY-0526-LY9359
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant EFNA5 protein, hFc Tag consist of Amino Acid: 1-202 and predicts a molecular mass of 48 kDa.

Molecule Mass

48 kDa

Sequence

Amino Acid: 1-202

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ephrin A5

Research Area

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.

Biological Process

Axon guidance Source: GO_Central
Ephrin receptor signaling pathway Source: UniProtKB
Negative regulation of substrate adhesion-dependent cell spreading Source: MGI
Nervous system development Source: ProtInc
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of protein phosphorylation Source: BHF-UCL
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cell-cell adhesion Source: UniProtKB
Regulation of cell morphogenesis Source: MGI
Regulation of focal adhesion assembly Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
Regulation of microtubule cytoskeleton organization Source: UniProtKB

Cellular Location

Cell membrane; Caveola. Compartmentalized in discrete caveolae-like membrane microdomains.

Xie, J., Xing, S., Shen, B. Y., Chen, H. T., Sun, B., Wang, Z. T., ... & Lu, X. X. (2021). PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth via regulating EFNA5. Human Cell, 34(2), 550-563.

Iguchi, T., Oka, Y., Yasumura, M., Omi, M., Kuroda, K., Yagi, H., ... & Sato, M. (2021). Mutually repulsive EphA7–EfnA5 organize region-to-region corticopontine projection by inhibiting collateral extension. Journal of Neuroscience, 41(22), 4795-4808.

Li, S., Hou, X., Wu, C., Han, L., Li, Q., Wang, J., & Luo, S. (2020). MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5. Biomedicine & Pharmacotherapy, 125, 109889.

Millner, T. O., Ricci, B., Zhang, X., Pomella, N., & Marino, S. (2019). Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma. Neuro-Oncology, 21(Suppl 4), iv1.

Pienkowski, V. M., Kucharczyk, M., Młynek, M., Szczałuba, K., Rydzanicz, M., Poszewiecka, B., ... & Płoski, R. (2019). Mapping of breakpoints in balanced chromosomal translocations by shallow whole-genome sequencing points to EFNA5, BAHD1 and PPP2R5E as novel candidates for genes causing human Mendelian disorders. Journal of Medical Genetics, 56(2), 104-112.

Jukonen, J. (2019). EfnA5 is a Non-Canonical Eph-Receptor Ligand and an Unfavorable Factor in High-Grade Serous Ovarian Cancer.

Zhou, Y., & Shiels, A. (2018). Epha2 and Efna5 participate in lens cell pattern-formation. Differentiation, 102, 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EFNA5 protein, hFc Tag (CAT#: V2LY-0526-LY3919)

Human Recombinant EFNA5 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY3920)

Human Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY3921)

Mouse Recombinant EFNA5 protein, hFc Tag (CAT#: V2LY-0526-LY8263)

Mouse Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY8264)

Rat Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY9360)

Rhesus Recombinant EFNA5 protein, hFc Tag (CAT#: V2LY-0526-LY9712)

Rhesus Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY9713)

Canine Recombinant Ephrin A5 protein, hFc Tag (CAT#: V2LY-0526-LY1237)

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Human Recombinant EFNA5 protein, hFc Tag (CAT#: V2LY-0526-LY3919)

Mouse Anti-EFNA5 Recombinant Antibody (CBFYE-0500) (CAT#: V2LY-0425-LY1518)

Human Recombinant EFNA5 protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY3920)

Rat Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY9360)

Mouse Recombinant EFNA5 protein, hFc Tag (CAT#: V2LY-0526-LY8263)

Mouse Anti-EFNA5 Recombinant Antibody (CBFYE-0501) (CAT#: V2LY-0425-LY1519)

Mouse Anti-EFNA5 Recombinant Antibody (CBFYE-0501) (CAT#: CBMAB-E0855-FY)

Canine Recombinant Ephrin A5 protein, hFc Tag (CAT#: V2LY-0526-LY1237)

Rhesus Recombinant EFNA5 protein, His Tag (CAT#: V2LY-0526-LY9713)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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