Mouse Anti-EHD4 Recombinant Antibody (1B5) (CBMAB-A2509-LY)

The product is antibody recognizes EHD4. The antibody 1B5 immunoassay techniques such as: WB, ELISA.
See all EHD4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1B5

Antibody Isotype

IgG1, κ

Application

WB, ELISA

Basic Information

Immunogen

EHD4 (AAH06287, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

EH-domain containing 4

Entrez Gene ID

30844

UniProt ID

Q9H223

Alternative Names

PAST4

Research Area

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.

Biological Process

Cellular response to growth factor stimulus Source: Ensembl
Cilium assembly Source: GO_Central
Endocytic recycling Source: UniProtKB
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Pinocytosis Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Protein homooligomerization Source: UniProtKB
Protein localization to plasma membrane Source: GO_Central
Regulation of endocytosis Source: Ensembl

Cellular Location

Early endosome membrane; Recycling endosome membrane; Cell membrane

References

Jones, T., Naslavsky, N., & Caplan, S. (2022). Differential requirements for the Eps15 homology Domain Proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic.

Malinova, T. S., Angulo-Urarte, A., Nüchel, J., Tauber, M., van Der Stoel, M. M., Janssen, V., ... & Huveneers, S. (2021). A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis. Nature communications, 12(1), 1-18.

Jones, T., Naslavsky, N., & Caplan, S. (2020). Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and fission. PloS one, 15(9), e0239657.

Lin, Y. S., Huang, K. Y., Yu, H. C., Lu, M. C., Fan, C. J., Tseng, H. Y. H., ... & Huang, H. B. (2020). Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. Biochemical and Biophysical Research Communications, 531(2), 236-241.

Dun, W., Danilo Jr, P., Mohler, P. J., & Boyden, P. A. (2018). Microtubular remodeling and decreased expression of Nav1. 5 with enhanced EHD4 in cells from the infarcted heart. Life sciences, 201, 72-80.

Rahman, S. (2018). Role of EPS15 Homology Domain-Containing Protein 4 (EHD4) in the Kidney.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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