Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176) (V2LY-0425-LY1601)

Name: Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176)
SKU: V2LY-0425-LY1601
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176) (V2LY-0425-LY1601-1) in WB and IP

Validation of mass spectrometric results.
Proteins identified by mass spectroscopy interacting with the wtPOC5 were analysed by CO-IP. Coimmunoprecipitation of myc-POC5 using anti-Myc antibodies in Hek293 cells. Proteins in the immune complexes were revealed by Western blotting with different antibodies as described in materials and methods. Control (cells transfected with Mock pCMV-entry vector) was used along cells transfected with myc tagged wt or POC5A429V expressing cells. A) Western blot on total cell lysate using POC5 antibody. Wt and POC5A429V tranfected cells have same expression levels of POC5. Mock transfected sample has very low expression of POC5. POC5 is observed at the expected size 63kDa in the wtPOC5 and at higher level in POC5A429V (indicated as p: phosphorylated). B) CO-IP shows the binding of Annexin2, Annexin 5, Desmocollin1 and CEP290, exclusively with wtPOC5. Very weak interaction was observed between POC5A429 with Acetylated-α-tubulin, Galectin3 and 7, EHD4, CystatinA compared to wtPOC5. ...View More

Hassan, A., Parent, S., Mathieu, H., Zaouter, C., Molidperee, S., Bagu, E. T., ... & Moldovan, F. (2019). Adolescent idiopathic scoliosis associated POC5 mutation impairs cell cycle, cilia length and centrosome protein interactions. PLoS One, 14(3), e0213269.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0176

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 331-372 mapping within an internal region of human EHD4.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Pig

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EH-domain containing 4

Entrez Gene ID

30844

UniProt ID

Q9H223

Research Area

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.

Biological Process

Cellular response to growth factor stimulus Source: Ensembl
Cilium assembly Source: GO_Central
Endocytic recycling Source: UniProtKB
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Pinocytosis Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
Protein homooligomerization Source: UniProtKB
Protein localization to plasma membrane Source: GO_Central
Regulation of endocytosis Source: Ensembl

Cellular Location

Early endosome membrane; Recycling endosome membrane; Cell membrane

Jones, T., Naslavsky, N., & Caplan, S. (2022). Differential requirements for the Eps15 homology Domain Proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic.

Malinova, T. S., Angulo-Urarte, A., Nüchel, J., Tauber, M., van Der Stoel, M. M., Janssen, V., ... & Huveneers, S. (2021). A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis. Nature communications, 12(1), 1-18.

Jones, T., Naslavsky, N., & Caplan, S. (2020). Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and fission. PloS one, 15(9), e0239657.

Lin, Y. S., Huang, K. Y., Yu, H. C., Lu, M. C., Fan, C. J., Tseng, H. Y. H., ... & Huang, H. B. (2020). Identification of phostensin in association with Eps 15 homology domain-containing protein 1 (EHD1) and EHD4. Biochemical and Biophysical Research Communications, 531(2), 236-241.

Dun, W., Danilo Jr, P., Mohler, P. J., & Boyden, P. A. (2018). Microtubular remodeling and decreased expression of Nav1. 5 with enhanced EHD4 in cells from the infarcted heart. Life sciences, 201, 72-80.

Rahman, S. (2018). Role of EPS15 Homology Domain-Containing Protein 4 (EHD4) in the Kidney.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176) (CAT#: CBMAB-E0417-FY)

Related Products

Mouse Anti-EHD4 Recombinant Antibody (1B5) (CAT#: V2LY-0425-LY1602)

Mouse Anti-EHD4 Recombinant Antibody (CBFYE-0176) (CAT#: CBMAB-E0417-FY)

Mouse Anti-EHD4 Recombinant Antibody (1B5) (CAT#: CBMAB-A2509-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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