Rabbit Anti-EI24 Recombinant Antibody (D3F6Z) (CBMAB-CP1968-LY)

The product is antibody recognizes EI24. The antibody D3F6Z immunoassay techniques such as: WB,IP.
See all EI24 antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

D3F6Z

Antibody Isotype

IgG

Application

WB, IP

Basic Information

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala31 of human EI24 protein.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

EI24, Autophagy Associated Transmembrane Protein

Introduction

This gene encodes a putative tumor suppressor and has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The encoded protein may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, 7, and 8. [provided by RefSeq, Feb 2014]

Entrez Gene ID

Human	9538
Mouse	13663
Rat	300514

UniProt ID

Human	O14681
Mouse	Q61070
Rat	Q4KM77

Alternative Names

EI24; Autophagy Associated Transmembrane Protein; P53-Induced Gene 8 Protein; Etoposide Induced 2.4; PIG8; Ectopic P-Granules Autophagy Protein 4 Homolog (C. Elegans); Ectopic P-Granules Autophagy Protein 4 Homolog;

Research Area

Acts as a negative growth regulator via p53-mediated apoptosis pathway. Regulates formation of degradative autolysosomes during autophagy (By similarity).

Biological Process

Apoptotic process Source: ProtInc
Macroautophagy Source: GO_Central
Negative regulation of cell growth Source: MGI

Cellular Location

Endoplasmic reticulum membrane; Nucleus membrane; Cytoplasm

Involvement in disease

EI24 is on a chromosomal region frequently deleted in solid tumors, and it is thought to play a role in breast and cervical cancer. Particularly, expression analysis of EI24 in cancerous tissues shows that EI24 loss is associated with tumor invasiveness.

References

Zhang, X., Mao, Y., Peng, W., Liu, H., Liang, L., Wang, D., ... & Zhang, X. (2021). Autophagy-related protein EI24 delays the development of pulmonary fibrosis by promoting autophagy. Life Sciences, 264, 118664.

Zhou, W., Yang, W., Yang, J., Zhu, H., Duan, L., Wang, X., ... & Hong, L. (2021). miR‑483 promotes the development of colorectal cancer by inhibiting the expression level of EI24. Molecular Medicine Reports, 24(2), 1-10.

Yuan, L., Liu, Q., Wang, Z., Hou, J., & Xu, P. (2020). EI24 tethers endoplasmic reticulum and mitochondria to regulate autophagy flux. Cellular and Molecular Life Sciences, 77(8), 1591-1606.

Zang, Y., Zhu, L., Li, T., Wang, Q., Li, J., Qian, Y., ... & Wang, L. (2018). EI24 Suppresses tumorigenesis in pancreatic cancer via regulating c-Myc. Gastroenterology research and practice, 2018.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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