Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0666) (CBMAB-E1083-FY)

Name: Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0666)
SKU: CBMAB-E1083-FY
Rating: 5 (1 reviews)

See all EIF2S1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0666) (CBMAB-E1083-FY-1) in WB

THP-1-derived macrophages were transfected with poly(I:C) (5 μg/ml for 2 h), or were treated with Nig (5 μM for 1 h) alone, or Nig together with Tg (5 μM for 1 h) as indicated. Cell extracts were probed for phosphorylated eIF2α (P-eIF2α; upper). ...View More

Yoshioka, D., Nakamura, T., Kubota, Y., & Takekawa, M. (2024). Formation of the NLRP3 inflammasome inhibits stress granule assembly by multiple mechanisms. The Journal of Biochemistry, 175(6), 629-641.

Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0666) (CBMAB-E1083-FY-2) in WB

Involvement of UPR. Panels (a-c) show whether or not UPR is involved, following treatment with PA and ALE in Huvec, MCF-7, and SH-SY5Y, respectively. The studied UPR sensor was PERK, and its possible activation was justified by the modulation of the expression of its phosphorylated form (p-PERK) and by the phosphorylation of eIF2α. ...View More

Maiuolo, J., Caminiti, R., Mazza, V., Oppedisano, F., Nucera, S., Serra, M., ... & Muscoli, C. (2025). An Innovative Method of Improving an Extract of Andrographis paniculata from Leaves: Its Anticancer Effect Involves the Cell Endoplasmic Reticulum. International Journal of Molecular Sciences, 26(1), 344.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0666

Application

ELISA, IF, IHC-P, WB, IP

Immunogen

Full length of human eIF2α.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 2 Subunit Alpha

Introduction

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987).

Entrez Gene ID

Human	1965
Mouse	13665
Rat	54318

UniProt ID

Human	P05198
Mouse	Q6ZWX6
Rat	P68101

Alternative Names

Eukaryotic Translation Initiation Factor 2 Subunit Alpha; Eukaryotic Translation Initiation Factor 2, Subunit 1 Alpha, 35kDa; EIF-2-Alpha; EIF-2alpha; EIF-2A; EIF2A

Research Area

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705).

This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:16289705).

Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PubMed:16289705).

In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PubMed:16289705).

EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352).

Biological Process

Aging Source: Ensembl
Cellular response to amino acid starvation Source: UniProtKB
Cellular response to heat Source: Ensembl
Cellular response to oxidative stress Source: ARUK-UCL
Cellular response to UV Source: UniProtKB
Negative regulation of guanyl-nucleotide exchange factor activity Source: Ensembl
Negative regulation of translational initiation in response to stress Source: UniProtKB
PERK-mediated unfolded protein response Source: UniProtKB
Positive regulation of neuron death Source: ParkinsonsUK-UCL
Positive regulation of type B pancreatic cell apoptotic process Source: Ensembl
Protein autophosphorylation Source: Ensembl
Response to endoplasmic reticulum stress Source: UniProtKB
Response to manganese-induced endoplasmic reticulum stress Source: Ensembl
Stress granule assembly Source: ARUK-UCL

Cellular Location

Stress granule. Colocalizes with NANOS3 in the stress granules.

PTM

Phosphorylation at Ser-49 and Ser-52 stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation, while concomitantly initiating the preferential translation of integrated stress response (ISR)-specific mRNAs (PubMed:15207627, PubMed:18032499, PubMed:19131336). Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2 (By similarity). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).
(Microbial infection) Phosphorylation by vaccinia virus protein E3 and rotavirus A stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation.

Li, Y., Shen, Y., Xie, M., Wang, B., Wang, T., Zeng, J., ... & Yang, M. (2022). LncRNAs LCETRL3 and LCETRL4 at chromosome 4q12 diminish EGFR-TKIs efficiency in NSCLC through stabilizing TDP43 and EIF2S1. Signal transduction and targeted therapy, 7(1), 1-12.

Hall, B. S., Dos Santos, S. J., Hsieh, L. T. H., Manifava, M., Ruf, M. T., Pluschke, G., ... & Simmonds, R. E. (2022). Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity. Autophagy, 18(4), 841-859.

Schatz, C., Sprung, S., Schartinger, V., Codina-Martínez, H., Lechner, M., Hermsen, M., & Haybaeck, J. (2021). Dysregulation of Translation Factors EIF2S1, EIF5A and EIF6 in Intestinal-Type Adenocarcinoma (ITAC). Cancers, 13(22), 5649.

Sun, P., Zhang, S., Qin, X., Chang, X., Cui, X., Li, H., ... & Li, Z. (2018). Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy, 14(2), 336-346.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0666)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-EIF2S1 Monoclonal Antibody (1C11) (CAT#: V2LY-0425-LY1666)

Mouse Anti-EIF2S1 Recombinant Antibody (CAP679) (CAT#: CBMAB-AP2030LY)

Mouse Anti-EIF2S1 Recombinant Antibody (CAP1030) (CAT#: CBMAB-AP2031LY)

Rat Anti-EIF2S1 Recombinant Antibody (L57A5) (CAT#: CBMAB-CP0558-LY)

Rabbit Anti-EIF2S1 (Phospho-S51) Recombinant Antibody (119A11) (CAT#: PTM-CBMAB-0264LY)

Mouse Anti-EIF2S1 Recombinant Antibody (11) (CAT#: CBMAB-A2527-LY)

Mouse Anti-EIF2S1 Monoclonal Antibody (1E2) (CAT#: CBMAB-0337-YC)

Mouse Anti-EIF2S1 Recombinant Antibody (CBFYE-0665) (CAT#: CBMAB-E1082-FY)

Mouse Anti-EIF2S1 Recombinant Antibody (2E4) (CAT#: CBMAB-E1079-FY)

Rabbit Anti-EIF2S1 (phospho-Ser51) Recombinant Antibody (CBNH-134) (CAT#: CBMAB-R4459-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry