Human Recombinant EIF2S1 protein, His Tag (V2LY-0526-LY3822)

Name: Human Recombinant EIF2S1 protein, His Tag
SKU: V2LY-0526-LY3822
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant EIF2S1 protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 40 kDa.

Molecule Mass

40 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tirs, NaCl, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 2 Subunit Alpha

Research Area

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705).

This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:16289705).

Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex (PubMed:16289705).

In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (PubMed:16289705).

EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352).

Biological Process

Aging Source: Ensembl
Cellular response to amino acid starvation Source: UniProtKB
Cellular response to heat Source: Ensembl
Cellular response to oxidative stress Source: ARUK-UCL
Cellular response to UV Source: UniProtKB
Negative regulation of guanyl-nucleotide exchange factor activity Source: Ensembl
Negative regulation of translational initiation in response to stress Source: UniProtKB
PERK-mediated unfolded protein response Source: UniProtKB
Positive regulation of neuron death Source: ParkinsonsUK-UCL
Positive regulation of type B pancreatic cell apoptotic process Source: Ensembl
Protein autophosphorylation Source: Ensembl
Response to endoplasmic reticulum stress Source: UniProtKB
Response to manganese-induced endoplasmic reticulum stress Source: Ensembl
Stress granule assembly Source: ARUK-UCL

Cellular Location

Stress granule. Colocalizes with NANOS3 in the stress granules.

PTM

Phosphorylation at Ser-49 and Ser-52 stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation, while concomitantly initiating the preferential translation of integrated stress response (ISR)-specific mRNAs (PubMed:15207627, PubMed:18032499, PubMed:19131336). Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2 (By similarity). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).
(Microbial infection) Phosphorylation by vaccinia virus protein E3 and rotavirus A stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation.

Li, Y., Shen, Y., Xie, M., Wang, B., Wang, T., Zeng, J., ... & Yang, M. (2022). LncRNAs LCETRL3 and LCETRL4 at chromosome 4q12 diminish EGFR-TKIs efficiency in NSCLC through stabilizing TDP43 and EIF2S1. Signal transduction and targeted therapy, 7(1), 1-12.

Hall, B. S., Dos Santos, S. J., Hsieh, L. T. H., Manifava, M., Ruf, M. T., Pluschke, G., ... & Simmonds, R. E. (2022). Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity. Autophagy, 18(4), 841-859.

Schatz, C., Sprung, S., Schartinger, V., Codina-Martínez, H., Lechner, M., Hermsen, M., & Haybaeck, J. (2021). Dysregulation of Translation Factors EIF2S1, EIF5A and EIF6 in Intestinal-Type Adenocarcinoma (ITAC). Cancers, 13(22), 5649.

Sun, P., Zhang, S., Qin, X., Chang, X., Cui, X., Li, H., ... & Li, Z. (2018). Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy, 14(2), 336-346.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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