Mouse Anti-EIF4E Recombinant Antibody (CBFYE-0709) (V2LY-0425-LY1732)

Name: Mouse Anti-EIF4E Recombinant Antibody (CBFYE-0709)
SKU: V2LY-0425-LY1732
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EIF4E Recombinant Antibody (CBFYE-0709) (V2LY-0425-LY1732-1) in WB

The protein levels for p-4EBP1, 4E-BP1, and eIF4E were assessed. GAPDH was the loading control. ...View More

Lee, M. K., Choi, J. W., Choi, Y. H., & Nam, T. J. (2019). Protective effect of Pyropia yezoensis peptide on dexamethasone-induced myotube atrophy in C2C12 myotubes. Marine Drugs, 17(5), 284.

Mouse Anti-EIF4E Recombinant Antibody (CBFYE-0709) (V2LY-0425-LY1732-2) in WB

The phosphorylation levels of mTOR, p70S6K and 4EBP1 in CCD-986sk cells were measured by Western blotting. ...View More

Liu, P., Choi, J. W., Lee, M. K., Choi, Y. H., & Nam, T. J. (2019). Wound healing potential of spirulina protein on CCD-986sk cells. Marine Drugs, 17(2), 130.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0709

Application

WB, IF, IP, IHC-P, ELISA

Immunogen

Amino acids 185-214 at the C-terminus of human Eif4e.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 4E

Entrez Gene ID

Human	1977
Mouse	13684
Rat	117045

UniProt ID

Human	P06730
Mouse	P63073
Rat	P63074

Research Area

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:16271312, PubMed:22578813).

In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm (PubMed:24335285).

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap (By similarity).

Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).

In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).

May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).

Biological Process

Behavioral fear response Source: Ensembl
Cellular response to dexamethasone stimulus Source: Ensembl
G1/S transition of mitotic cell cycle Source: UniProtKB
Lung development Source: Ensembl
Negative regulation of neuron differentiation Source: Ensembl
Negative regulation of translation Source: Ensembl
Positive regulation of mitotic cell cycle Source: UniProtKB
Regulation of translation Source: UniProtKB
Regulation of translation at postsynapse, modulating synaptic transmission Source: Ensembl
Stem cell population maintenance Source: Ensembl

Cellular Location

Nucleus; Cytoplasm; P-body; Stress granule. Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732). Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257).

Involvement in disease

Autism 19 (AUTS19):
Disease susceptibility is associated with variants affecting the gene represented in this entry. A heterozygous single-nucleotide insertion has been found in families affected by autism. The variant results in increased promoter activity and is involved in disease pathogenesis through EIF4E deregulation (PubMed:19556253). A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. A chromosomal aberration involving EIF4E has been found in a patient with classic autism. Translocation t(45)(q23q31.3). The breakpoint on chromosome 4 is located 56 kb downstream of EIF4E (PubMed:19556253).

PTM

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.

Krause, L., Willing, F., Andreou, A. Z., & Klostermeier, D. (2022). The domains of yeast eIF4G, eIF4E and the cap fine-tune eIF4A activities through an intricate network of stimulatory and inhibitory effects. Nucleic acids research, 50(11), 6497-6510.

Conn, C. S., Yang, H., Tom, H. J., Ikeda, K., Oses-Prieto, J. A., Vu, H., ... & Ruggero, D. (2021). The major cap-binding protein eIF4E regulates lipid homeostasis and diet-induced obesity. Nature Metabolism, 3(2), 244-257.

Romagnoli, A., D’Agostino, M., Ardiccioni, C., Maracci, C., Motta, S., La Teana, A., & Di Marino, D. (2021). Control of the eIF4E activity: structural insights and pharmacological implications. Cellular and Molecular Life Sciences, 78(21), 6869-6885.

Aguilar-Valles, A., De Gregorio, D., Matta-Camacho, E., Eslamizade, M. J., Khlaifia, A., Skaleka, A., ... & Sonenberg, N. (2021). Antidepressant actions of ketamine engage cell-specific translation via eIF4E. Nature, 590(7845), 315-319.

Knight, J. R., Alexandrou, C., Skalka, G. L., Vlahov, N., Pennel, K., Officer, L., ... & Sansom, O. J. (2021). MNK Inhibition Sensitizes KRAS-Mutant Colorectal Cancer to mTORC1 Inhibition by Reducing eIF4E Phosphorylation and c-MYC ExpressionTargeting P-eIF4E and mTORC1 in KRAS-Mutant Colorectal Cancer. Cancer discovery, 11(5), 1228-1247.

Yang, X., Zhong, W., & Cao, R. (2020). Phosphorylation of the mRNA cap-binding protein eIF4E and cancer. Cellular signalling, 73, 109689.

Wan, X., Yang, T., Cuesta, A., Pang, X., Balius, T. E., Irwin, J. J., ... & Taunton, J. (2020). Discovery of lysine-targeted eIF4E inhibitors through covalent docking. Journal of the American Chemical Society, 142(11), 4960-4964.

Culjkovic-Kraljacic, B., Skrabanek, L., Revuelta, M. V., Gasiorek, J., Cowling, V. H., Cerchietti, L., & Borden, K. L. (2020). The eukaryotic translation initiation factor eIF4E elevates steady-state m7G capping of coding and noncoding transcripts. Proceedings of the National Academy of Sciences, 117(43), 26773-26783.

Coutinho de Oliveira, L., Volpon, L., Rahardjo, A. K., Osborne, M. J., Culjkovic-Kraljacic, B., Trahan, C., ... & Borden, K. L. (2019). Structural studies of the eIF4E–VPg complex reveal a direct competition for capped RNA: Implications for translation. Proceedings of the National Academy of Sciences, 116(48), 24056-24065.

Amorim, I. S., Lach, G., & Gkogkas, C. G. (2018). The role of the eukaryotic translation initiation factor 4E (eIF4E) in neuropsychiatric disorders. Frontiers in genetics, 9, 561.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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