Mouse Anti-ELANE Recombinant Antibody (CBWJN-0901) (CBMAB-N0022-WJ)

Name: Mouse Anti-ELANE Recombinant Antibody (CBWJN-0901)
SKU: CBMAB-N0022-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-0901

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 251-267 of human Neutrophil Elastase.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Elastase, Neutrophil Expressed

Introduction

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Entrez Gene ID

1991

UniProt ID

P08246

Alternative Names

Elastase, Neutrophil Expressed; Medullasin; Bone Marrow Serine Protease; Elastase 2, Neutrophil; Neutrophil Elastase; Leukocyte Elastase; PMN Elastase; Elastase-2; ELA2; HLE; Granulocyte-Derived Elastase;

Research Area

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022).

Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).

Biological Process

Acute inflammatory response to antigenic stimulus Source: GO_Central
Biosynthetic process of antibacterial peptides active against Gram-negative bacteria Source: UniProtKB
Cellular calcium ion homeostasis Source: UniProtKB
Defense response to bacterium Source: UniProtKB
Extracellular matrix disassembly Source: Reactome
Leukocyte migration involved in inflammatory response Source: Ensembl
Negative regulation of chemokine production Source: UniProtKB
Negative regulation of chemotaxis Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of interleukin-8 production Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Neutrophil-mediated killing of fungus Source: Ensembl
Neutrophil-mediated killing of gram-negative bacterium Source: UniProtKB
Phagocytosis Source: GO_Central
Positive regulation of immune response Source: Ensembl
Positive regulation of interleukin-8 production Source: UniProtKB
Positive regulation of leukocyte tethering or rolling Source: Ensembl
Positive regulation of MAP kinase activity Source: UniProtKB
Positive regulation of smooth muscle cell proliferation Source: UniProtKB
Protein catabolic process Source: UniProtKB
Proteolysis Source: UniProtKB
Pyroptosis Source: Reactome
Response to lipopolysaccharide Source: Ensembl
Response to UV Source: UniProtKB
Response to yeast Source: Ensembl

Cellular Location

Phagosome. Localized in phagolysosomes following ingestion of E.coli by neutrophils.

Involvement in disease

Cyclic haematopoiesis (CH):
Autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Neutropenia, severe congenital 1, autosomal dominant (SCN1):
A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.

Rao, S., Yao, Y., de Brito, J. S., Yao, Q., Shen, A. H., Watkinson, R. E., ... & Bauer, D. E. (2021). Dissecting ELANE neutropenia pathogenicity by human HSC gene editing. Cell Stem Cell, 28(5), 833-845.

Saha, R., Pradhan, S. S., Das, P., Mishra, P., Singh, R., Sivaramakrishnan, V., & Acharya, P. (2021). Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports, 11(1), 1-16.

Tran, N. T., Graf, R., Wulf-Goldenberg, A., Stecklum, M., Strauß, G., Kühn, R., ... & Rajewsky, K. (2020). CRISPR-Cas9-mediated ELANE mutation correction in hematopoietic stem and progenitor cells to treat severe congenital neutropenia. Molecular Therapy, 28(12), 2621-2634.

Dale, D. C., & Makaryan, V. (2018). ELANE-related neutropenia.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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