Mouse Anti-ELOA Recombinant Antibody (CBYJT-1213) (CBMAB-T0227-YJ)

Name: Mouse Anti-ELOA Recombinant Antibody (CBYJT-1213)
SKU: CBMAB-T0227-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1213

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-300 mapping at the N-terminus of human Elongin A.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Elongin A

Introduction

ELOA is the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. ELOA activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A plays a role as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Entrez Gene ID

6924

UniProt ID

Q14241

Alternative Names

Elongin A; Transcription Elongation Factor B (SIII), Polypeptide 3 (110kDa, Elongin A); RNA Polymerase II Transcription Factor SIII Subunit A1; Transcription Elongation Factor B Polypeptide 3; Transcription Elongation Factor B Subunit 3; Elongin 110 KDa Subunit; SIII P110

Biological Process

Regulation of transcription by RNA polymerase II Source: ProtInc
Transcription elongation from RNA polymerase II promoter Source: InterPro

Cellular Location

Nucleus

Hu, L., Zhang, W., Xiang, Z., Wang, Y., Zeng, C., Wang, X., ... & Chen, L. (2022). EloA promotes HEL polyploidization upon PMA stimulation through enhanced ERK1/2 activity. Platelets, 33(5), 755-763.

Ardehali, M. B., Damle, M., Perea-Resa, C., Blower, M. D., & Kingston, R. E. (2021). Elongin A associates with actively transcribed genes and modulates enhancer RNA levels with limited impact on transcription elongation rate in vivo. Journal of Biological Chemistry, 296.

Wang, Y., Hou, L., Ardehali, M. B., Kingston, R. E., & Dynlacht, B. D. (2021). Elongin A regulates transcription in vivo through enhanced RNA polymerase processivity. Journal of Biological Chemistry, 296.

Sanchez, G., Barbier, J., Elie, C., Kiernan, R., & Rouquier, S. (2020). PAF1 facilitates RNA polymerase II ubiquitination by the Elongin A complex through phosphorylation by CDK12. bioRxiv.

Weems, J. C., Conaway, J., & Conaway, R. (2019). Elongin and the Elongin A ubiquitin ligase complex in transcription and the response to DNA damage. The FASEB Journal, 33(S1), 457-28.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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