Mouse Anti-EME1 Recombinant Antibody (XB0392) (V2LY-0825-LY160)

Name: Mouse Anti-EME1 Recombinant Antibody (XB0392)
SKU: V2LY-0825-LY160
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EME1 Recombinant Antibody (XB0392) (V2LY-0825-LY160) in WB

FIBP positively regulates EME1 protein expression in lung adenocarcinoma cells. ...View More

Xu, Y., Li, J., Zhu, K., Zeng, Y., Chen, J., Dong, X., ... & Wu, G. (2023). FIBP interacts with transcription factor STAT3 to induce EME1 expression and drive radioresistance in lung adenocarcinoma. International Journal of Biological Sciences, 19(12), 3816.

Mouse Anti-EME1 Recombinant Antibody (XB0392) (V2LY-0825-LY160) in WB

Western blot analysis of the expression of EME1 in lung adenocarcinoma cells after transfection with the indicated plasmids or siRNAs. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

XB0392

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 37-91 mapping near the N-terminus of mouse Eme1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Essential Meiotic Structure-Specific Endonuclease 1

Entrez Gene ID

146956

UniProt ID

Q96AY2

Research Area

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Biological Process

Double-strand break repair Source: GO_Central
Mitotic intra-S DNA damage checkpoint signaling Source: GO_Central
Replication fork processing Source: GO_Central
Resolution of meiotic recombination intermediates Source: GO_Central
Response to intra-S DNA damage checkpoint signaling Source: MGI

Cellular Location

Nucleolus. Recruited to regions of DNA damage in S-phase cells.

Giaccherini, C., Scaglione, S., Coulon, S., Dehé, P. M., & Gaillard, P. H. L. (2022). Regulation of Mus81-Eme1 structure-specific endonuclease by Eme1 SUMO-binding and Rad3ATR kinase is essential in the absence of Rqh1BLM helicase. PLoS genetics, 18(4), e1010165.

Elango, R., Panday, A., Lach, F. P., Willis, N. A., Nicholson, K., Duffey, E. E., ... & Scully, R. (2022). The structure-specific endonuclease complex SLX4–XPF regulates Tus–Ter-induced homologous recombination. Nature Structural & Molecular Biology, 29(8), 801-812.

Guo, Z., Liang, E., Li, W., Jiang, L., & Zhi, F. (2021). Essential meiotic structure-specific endonuclease1 (EME1) promotes malignant features in gastric cancer cells via the Akt/GSK3B/CCND1 pathway. Bioengineered, 12(2), 9869-9884.

Giaccherini, C., & Gaillard, P. H. L. (2021). Control of structure-specific endonucleases during homologous recombination in eukaryotes. Current Opinion in Genetics & Development, 71, 195-205.

Kim, S. M., & Forsburg, S. L. (2018). Regulation of structure-specific endonucleases in replication stress. Genes, 9(12), 634.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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