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Mouse Anti-ENC1 Recombinant Antibody (3B1) (CBMAB-A2605-LY)

The product is antibody recognizes ENC1. The antibody 3B1 immunoassay techniques such as: WB, ELISA.
See all ENC1 antibodies
Published Data
Fig. 1 Western blotting analysis of RALBP1, β-catenin, ENC1, and β-actin after 24 h treatment with PKF115-584 or PNU74654 in H295R cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3B1
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
ENC1 (NP_003624, 17 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ectodermal-neural cortex (with BTB-like domain)
Introduction
DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 oCcur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM
Entrez Gene ID
8507
UniProt ID
O14682
Alternative Names
CCL28; ENC-1; FLJ39259; KLHL35; KLHL37; NRPB; PIG10; TP53I10
Research Area
Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism.
Biological Process
Multicellular organism development Source: ProtInc
Negative regulation of translation Source: UniProtKB
Nervous system development Source: ProtInc
Positive regulation of neuron projection development Source: Ensembl
Proteasomal ubiquitin-independent protein catabolic process Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Cellular Location
Nucleus matrix; Cytoskeleton; Cytoplasm
PTM
Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination.

Zhang, P., Zhao, F., Jia, K., & Liu, X. (2022). The LOXL1 antisense RNA 1 (LOXL1-AS1)/microRNA-423-5p (miR-423-5p)/ectodermal-neural cortex 1 (ENC1) axis promotes cervical cancer through the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway. Bioengineered, 13(2), 2567-2584.

Mamoor, S. (2022). Differential expression of ENC1 in cancers of the breast.

Li, X., Zhao, H., Liu, J., & Tong, J. (2021). Long Non-coding RNA MIAT knockdown prevents the formation of intracranial aneurysm by downregulating ENC1 via MYC. Frontiers in physiology, 11, 572605.

Wu, C., Wang, X., Wu, X., & Chen, X. (2021). Ectodermal‑neural cortex 1 affects the biological function of lung cancer through the MAPK pathway. International Journal of Molecular Medicine, 47(5), 1-12.

Mamoor, S. (2021). Differential expression of ectodermal-neural cortex 1 in human epithelial ovarian cancer.

Li, L., Wang, N., Zhu, M., Xiong, Y., Wang, F., Guo, G., ... & Gu, Y. (2021). Aberrant super-enhancer-driven oncogene ENC1 promotes the radio-resistance of breast carcinoma. Cell death & disease, 12(8), 1-13.

Cui, Y., Yang, J., Bai, Y., Li, Q., Yao, Y., Liu, C., ... & Zhang, Y. (2021). ENC1 facilitates colorectal carcinoma tumorigenesis and metastasis via JAK2/STAT5/AKT axis-mediated epithelial mesenchymal transition and stemness. Frontiers in cell and developmental biology, 9, 616887.

Zhou, Y., Tang, X., Niu, L., Liu, Y., Wang, B., & He, J. (2020). Ectodermal‐neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β‐catenin pathway. Journal of cellular and molecular medicine, 24(15), 8826-8835.

Fan, S., Wang, Y., Sheng, N., Xie, Y., Lu, J., Zhang, Z., ... & Zheng, Y. (2019). Low expression of ENC1 predicts a favorable prognosis in patients with ovarian cancer. Journal of Cellular Biochemistry, 120(1), 861-871.

Pereira, T. F. (2018). Role of Ectodermal-neural cortex 1 protein in human glioma progression, identification of a peptide internalized by human glioblastoma cells and development of an alternative method to generate growth curves of adherent cultures (Doctoral dissertation, Universidade de São Paulo).

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For research use only. Not intended for any clinical use.

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