Mouse Anti-ENC1 Recombinant Antibody (CBFYE-0212) (CBMAB-E0460-FY)

Name: Mouse Anti-ENC1 Recombinant Antibody (CBFYE-0212)
SKU: CBMAB-E0460-FY
Rating: 5 (1 reviews)

See all ENC1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-ENC1 Recombinant Antibody (CBFYE-0212) (CBMAB-E0460-FY) in IHC

Immunohistochemical staining was performed to detect ENC1 expression in breast cancer with different grades (low grade: LG, high grade: HG) and normal breast specimens. Representative ENC1 staining was shown, and IOD value was used to quantify the results. The experiments were performed and calculated in triplicate (N = 3). Scale bars, 200 µm. ...View More

Zhou, Y., Tang, X., Niu, L., Liu, Y., Wang, B., & He, J. (2020). Ectodermal-neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β-catenin pathway. Journal of Cellular and Molecular Medicine, 24(15), 8826-8835.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0212

Application

WB, IF, IP

Immunogen

Amino acids 413-524 of mouse ENC1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:50-1:500
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 17-98

More Infomation

Target

Full Name

ectodermal-neural cortex (with BTB-like domain)

Introduction

This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Entrez Gene ID

8507

UniProt ID

O14682

Alternative Names

Ectodermal-Neural Cortex 1; Ectodermal-Neural Cortex 1 (With BTB-Like Domain); Ectodermal-Neural Cortex 1 (With BTB Domain); Nuclear Matrix Protein NRP/B; Kelch-Like Family Member 37; P53-Induced Gene 10 Protein; Kelch-Like Protein 37; KLHL37; ENC-1; PIG10

Research Area

Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism.

Biological Process

Multicellular organism development Source: ProtInc
Negative regulation of translation Source: UniProtKB
Nervous system development Source: ProtInc
Positive regulation of neuron projection development Source: Ensembl
Proteasomal ubiquitin-independent protein catabolic process Source: UniProtKB
Protein ubiquitination Source: UniProtKB

Cellular Location

Nucleus matrix; Cytoskeleton; Cytoplasm

PTM

Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination.

Zhang, P., Zhao, F., Jia, K., & Liu, X. (2022). The LOXL1 antisense RNA 1 (LOXL1-AS1)/microRNA-423-5p (miR-423-5p)/ectodermal-neural cortex 1 (ENC1) axis promotes cervical cancer through the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway. Bioengineered, 13(2), 2567-2584.

Mamoor, S. (2022). Differential expression of ENC1 in cancers of the breast.

Li, X., Zhao, H., Liu, J., & Tong, J. (2021). Long Non-coding RNA MIAT knockdown prevents the formation of intracranial aneurysm by downregulating ENC1 via MYC. Frontiers in physiology, 11, 572605.

Wu, C., Wang, X., Wu, X., & Chen, X. (2021). Ectodermal‑neural cortex 1 affects the biological function of lung cancer through the MAPK pathway. International Journal of Molecular Medicine, 47(5), 1-12.

Mamoor, S. (2021). Differential expression of ectodermal-neural cortex 1 in human epithelial ovarian cancer.

Li, L., Wang, N., Zhu, M., Xiong, Y., Wang, F., Guo, G., ... & Gu, Y. (2021). Aberrant super-enhancer-driven oncogene ENC1 promotes the radio-resistance of breast carcinoma. Cell death & disease, 12(8), 1-13.

Cui, Y., Yang, J., Bai, Y., Li, Q., Yao, Y., Liu, C., ... & Zhang, Y. (2021). ENC1 facilitates colorectal carcinoma tumorigenesis and metastasis via JAK2/STAT5/AKT axis-mediated epithelial mesenchymal transition and stemness. Frontiers in cell and developmental biology, 9, 616887.

Zhou, Y., Tang, X., Niu, L., Liu, Y., Wang, B., & He, J. (2020). Ectodermal‐neural cortex 1 as a novel biomarker predicts poor prognosis and induces metastasis in breast cancer by promoting Wnt/β‐catenin pathway. Journal of cellular and molecular medicine, 24(15), 8826-8835.

Fan, S., Wang, Y., Sheng, N., Xie, Y., Lu, J., Zhang, Z., ... & Zheng, Y. (2019). Low expression of ENC1 predicts a favorable prognosis in patients with ovarian cancer. Journal of Cellular Biochemistry, 120(1), 861-871.

Pereira, T. F. (2018). Role of Ectodermal-neural cortex 1 protein in human glioma progression, identification of a peptide internalized by human glioblastoma cells and development of an alternative method to generate growth curves of adherent cultures (Doctoral dissertation, Universidade de São Paulo).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ENC1 Recombinant Antibody (CBFYE-0212)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ENC1 Recombinant Antibody (3B1) (CAT#: CBMAB-A2605-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry