Mouse Anti-ERCC6L Recombinant Antibody (3F12-2B10) (V2LY-0825-LY676)

Name: Mouse Anti-ERCC6L Recombinant Antibody (3F12-2B10)
SKU: V2LY-0825-LY676
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ERCC6L Recombinant Antibody (3F12-2B10) (V2LY-0825-LY676) in IF

Representative immunofluorescence deconvoluted z-projection images of PICH-positive UFBs in HeLaanaphase cells. DNA was visualized by DAPI staining (blue) and UFBs were stained with PICH antibody (in green). Enlarged image correspond to the yellowsquare. Scale bar: 5 µm ...View More

Gemble, S., Buhagiar-Labarchède, G., Onclercq-Delic, R., Fontaine, G., Lambert, S., & Amor-Guéret, M. (2020). Topoisomerase IIα prevents ultrafine anaphase bridges by two mechanisms. Open Biology, 10(5), 190259.

Mouse Anti-ERCC6L Recombinant Antibody (3F12-2B10) (V2LY-0825-LY676) in IF

a, b Representative immunofluorescence (IF) z-projection images showing paired Tau foci (green) linked by PICH-positive UFBs in BS (a) and HeLa (b) anaphase cells. Scale bar: 5 µm. DNA was visualized by DAPI staining (white). Centromeres were stained with CREST serum (blue) and UFBs were stained with PICH antibody (red). In the enlarged images, Tau foci at the extremities of UFBs are indicated by yellow arrows. ...View More

Bou Samra, E., Buhagiar-Labarchède, G., Machon, C., Guitton, J., Onclercq-Delic, R., Green, M. R., ... & Amor-Guéret, M. (2017). A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nature communications, 8(1), 693.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3F12-2B10

Application

ELISA, WB

Immunogen

Full length recombinant ERCC6L.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ERCC Excision Repair 6 Like, Spindle Assembly Checkpoint Helicase

Entrez Gene ID

54821

UniProt ID

Q2NKX8

Research Area

DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed:17218258, PubMed:23973328).

Functions as ATP-dependent DNA translocase (PubMed:23973328, PubMed:28977671).

Can promote Holliday junction branch migration (in vitro) (PubMed:23973328).

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW

Cellular Location

Centromere; Kinetochore; Chromosome. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.

PTM

Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

Huang, X., Jiang, L., Lu, S., Yuan, M., Lin, H., Li, B., ... & Zhong, Y. (2022). Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. Oncology Reports, 48(1), 1-18.

Hou, G., Lu, Z., Bi, Y., Deng, J., & Yang, X. (2022). ERCC6L is a biomarker and therapeutic target for non–small cell lung adenocarcinoma. Medical Oncology, 39(5), 1-16.

Zhang, G., Yu, Z., Fu, S., Lv, C., Dong, Q., Fu, C., ... & Zeng, Y. (2022). Correction to: ERCC6L that is up-regulated in high grade of renal cell carcinoma enhances cell viability in vitro and promotes tumor growth in vivo potentially through modulating MAPK signalling pathway. Cancer Gene Therapy, 1-1.

Zhang, G., Ma, J., Xiong, J., Huang, X., Han, X., Yu, X., & Jiang, X. (2021). Upregulation of Excision Repair Cross-Complementation Group 6-Like (ERCC6L) Promotes Tumor Growth in Hepatocellular Carcinoma. Digestive Diseases and Sciences, 66(4), 1097-1109.

Chen, D., Liu, Q., & Cao, G. (2021). ERCC6L promotes cell growth and metastasis in gastric cancer through activating NF-κB signaling. Aging (Albany NY), 13(16), 20218.

Yu, B., Liang, H., Ye, Q., & Wang, Y. (2020). Upregulation of ERCC6L is associated with tumor progression and unfavorable prognosis in hepatocellular carcinoma. Journal of Gastrointestinal Oncology, 11(5), 1009.

Chen, H., Wang, H., Yu, X., Zhou, S., Zhang, Y., Wang, Z., ... & Wang, Z. (2020). ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway. BMC cancer, 20(1), 1-10.

Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., & Qi, J. (2019). ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncology letters, 18(1), 237-246.

Zhang, G., Yu, Z., Fu, S., Lv, C., Dong, Q., Fu, C., ... & Zeng, Y. (2019). ERCC6L that is up-regulated in high grade of renal cell carcinoma enhances cell viability in vitro and promotes tumor growth in vivo potentially through modulating MAPK signalling pathway. Cancer Gene Therapy, 26(9), 323-333.

Liu, J., Sun, J., Zhang, Q., & Zeng, Z. (2018). shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Molecular Medicine Reports, 18(3), 3490-3496.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ERCC6L Matched Antibody Pair (388) (CAT#: APMAB-388LY)

Related Products

Rabbit Anti-ERCC6L Recombinant Antibody (P0316) (CAT#: V2LY-0825-LY677)

ERCC6L Matched Antibody Pair (388) (CAT#: APMAB-388LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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