Mouse Anti-ESRRA Recombinant Antibody (CAP725) (CBMAB-AP2153LY)

Name: Mouse Anti-ESRRA Recombinant Antibody (CAP725)
SKU: CBMAB-AP2153LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ESRRA Recombinant Antibody (CAP725) (CBMAB-AP2153LY) in IP

Interaction between HIF-1α and ERRα was verified using the co-immunoprecipitation (Co-IP) analysis of HEC-1A−ovERRα cell lysates. The corresponding antibodies (out-put IP: anti-HIF-1α-antibody and anti-ERRα-antibody) could precipitate both ERRα and HIF-1α, respectively, in the HEC-1A−ovERRα cell lysates. ...View More

Su, P., Mao, X., Ma, J., Huang, L., Yu, L., Tang, S., ... & Sun, P. (2023). ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. Journal of Experimental & Clinical Cancer Research, 42(1), 274.

Mouse Anti-ESRRA Recombinant Antibody (CAP725) (CBMAB-AP2153LY) in WB

Salidroside elevated the expression of ATGL protein in Ang II-stimulated H9C2 cellsH9C2 cells were treated with or without salidroside (100 μM) and stimulated with or without Ang II (1 × 10−7 M) for 24 h. The protein expressions of HSF-1, ATGL, ERRα, and RXRα were detected by Western blotting. Experiments have been repeated four times. The values represent as means ± SD. *p < 0.05. ...View More

Gao, H., Tian, K., Meng, Y., Liu, X., & Peng, Y. (2022). Salidroside ameliorates cardiomyocyte hypertrophy by upregulating peroxisome proliferator-activated receptor-α. Frontiers in Pharmacology, 13, 865434.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CAP725

Application

IP, WB

Immunogen

Amino acids 198-376 of human ERRα.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Estrogen Related Receptor Alpha

Introduction

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Entrez Gene ID

Human	2101
Rat	293701

UniProt ID

Human	P11474
Rat	Q5QJV7

Alternative Names

Estrogen Related Receptor Alpha; Nuclear Receptor Subfamily 3 Group B Member 1; Estrogen Receptor-Like 1; ERR-Alpha; ESRL1; NR3B1; ERR1;

Research Area

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

Biological Process

Cartilage development Source: Ensembl
Positive regulation of cellular response to insulin stimulus Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of cell population proliferation Source: Ensembl
Regulation of ossification Source: Ensembl
Regulation of osteoblast differentiation Source: Ensembl
Regulation of osteoclast differentiation Source: Ensembl
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to estradiol Source: Ensembl

Cellular Location

Cytoplasm; Nucleus. Co-localizes to the cytoplasm only in presence of MAPK15.

PTM

Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated with SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activity, but has no effect on subcellular location nor on DNA binding.
Reversibly acetylated. Acetylation by PCAF/KAT2 at Lys-129, Lys-138, Lys-160 and Lys-162 and PCAF/KAT2 decreases transcriptional activity probably by inhibiting DNA-binding activity; deacetylation involves SIRT1 and HDAC8 and increases DNA-binding.

Li, F. N., Zhang, Q. Y., Li, O., Liu, S. L., Yang, Z. Y., Pan, L. J., ... & Dong, P. (2021). ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. International journal of biological sciences, 17(8), 1909.

Bergeron, A., MacGrogan, G., Bertaut, A., Ladoire, S., Arveux, P., Desmoulins, I., ... & Arnould, L. (2021). Triple-negative breast lobular carcinoma: a luminal androgen receptor carcinoma with specific ESRRA mutations. Modern Pathology, 34(7), 1282-1296.

Dhillon, P., Park, J., Del Pozo, C. H., Li, L., Doke, T., Huang, S., ... & Susztak, K. (2021). The nuclear receptor ESRRA protects from kidney disease by coupling metabolism and differentiation. Cell metabolism, 33(2), 379-394.

Kim, S., Lee, J. Y., Shin, S. G., Kim, J. K., Silwal, P., Kim, Y. J., ... & Jo, E. K. (2021). ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota. Autophagy, 17(10), 2856-2875.

Ismael, W. M., Gao, M., & Yemeni, Z. (2021). ESRRA-IoT: Edge-based spatial redundancy reduction approach for Internet of Things. Internet of Things, 14, 100388.

Kan, J., Hu, Y., Ge, Y., Zhang, W., Lu, S., Zhao, C., ... & Liu, Y. (2021). Declined expressions of vast mitochondria-related genes represented by CYCS and transcription factor ESRRA in skeletal muscle aging. Bioengineered, 12(1), 3485-3502.

Wang, S., & Huo, X. (2021). Comprehensive analysis of ESRRA in endometrial cancer. Technology in Cancer Research & Treatment, 20, 1533033821992083.

Huang, X. (2020). 387 Estrogen-related receptor alpha (ESRRA) copy number variation is associated with histological grade in ovarian cancer. International Journal of Gynecological Cancer, 30(Suppl 3), A161-A161.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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