Mouse Anti-ESRRA Recombinant Antibody (CBFYE-1300) (V2LY-0825-LY809)

Name: Mouse Anti-ESRRA Recombinant Antibody (CBFYE-1300)
SKU: V2LY-0825-LY809
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ESRRA Recombinant Antibody (CBFYE-1300) (V2LY-0825-LY809) in WB

The expression of IGF2BP1 and ERRα in sh-Control and sh-IGF2BP1 Dox-resistant OS cells were checked by western blot analysis. ...View More

He, Q., Hao, P., He, G., Mai, H., Liu, W., Zhang, W., ... & Chen, Y. (2022). IGF2BP1-regulated expression of ERRα is involved in metabolic reprogramming of chemotherapy resistant osteosarcoma cells. Journal of Translational Medicine, 20(1), 348.

Mouse Anti-ESRRA Recombinant Antibody (CBFYE-1300) (V2LY-0825-LY809) in WB

The protein expression of ERRα in OS chemoresistant or parental cells were checked by western blot analysis. ...View More

Chen, Y., Zhang, K., Li, Y., Guo, R., Zhang, K., Zhong, G., & He, Q. (2019). Oestrogen-related receptor alpha mediates chemotherapy resistance of osteosarcoma cells via regulation of ABCB 1. Journal of Cellular and Molecular Medicine, 23(3), 2115-2124.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1300

Application

IP, WB

Immunogen

Amino acids 1-78 of human ERRα.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Estrogen Related Receptor Alpha

Entrez Gene ID

Human	2101
Rat	293701

UniProt ID

Human	P11474
Rat	Q5QJV7

Research Area

Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.

Biological Process

Cartilage development Source: Ensembl
Positive regulation of cellular response to insulin stimulus Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of cell population proliferation Source: Ensembl
Regulation of ossification Source: Ensembl
Regulation of osteoblast differentiation Source: Ensembl
Regulation of osteoclast differentiation Source: Ensembl
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to estradiol Source: Ensembl

Cellular Location

Cytoplasm; Nucleus. Co-localizes to the cytoplasm only in presence of MAPK15.

PTM

Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated with SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activity, but has no effect on subcellular location nor on DNA binding.
Reversibly acetylated. Acetylation by PCAF/KAT2 at Lys-129, Lys-138, Lys-160 and Lys-162 and PCAF/KAT2 decreases transcriptional activity probably by inhibiting DNA-binding activity; deacetylation involves SIRT1 and HDAC8 and increases DNA-binding.

Li, F. N., Zhang, Q. Y., Li, O., Liu, S. L., Yang, Z. Y., Pan, L. J., ... & Dong, P. (2021). ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. International journal of biological sciences, 17(8), 1909.

Bergeron, A., MacGrogan, G., Bertaut, A., Ladoire, S., Arveux, P., Desmoulins, I., ... & Arnould, L. (2021). Triple-negative breast lobular carcinoma: a luminal androgen receptor carcinoma with specific ESRRA mutations. Modern Pathology, 34(7), 1282-1296.

Dhillon, P., Park, J., Del Pozo, C. H., Li, L., Doke, T., Huang, S., ... & Susztak, K. (2021). The nuclear receptor ESRRA protects from kidney disease by coupling metabolism and differentiation. Cell metabolism, 33(2), 379-394.

Kim, S., Lee, J. Y., Shin, S. G., Kim, J. K., Silwal, P., Kim, Y. J., ... & Jo, E. K. (2021). ESRRA (estrogen related receptor alpha) is a critical regulator of intestinal homeostasis through activation of autophagic flux via gut microbiota. Autophagy, 17(10), 2856-2875.

Ismael, W. M., Gao, M., & Yemeni, Z. (2021). ESRRA-IoT: Edge-based spatial redundancy reduction approach for Internet of Things. Internet of Things, 14, 100388.

Kan, J., Hu, Y., Ge, Y., Zhang, W., Lu, S., Zhao, C., ... & Liu, Y. (2021). Declined expressions of vast mitochondria-related genes represented by CYCS and transcription factor ESRRA in skeletal muscle aging. Bioengineered, 12(1), 3485-3502.

Wang, S., & Huo, X. (2021). Comprehensive analysis of ESRRA in endometrial cancer. Technology in Cancer Research & Treatment, 20, 1533033821992083.

Huang, X. (2020). 387 Estrogen-related receptor alpha (ESRRA) copy number variation is associated with histological grade in ovarian cancer. International Journal of Gynecological Cancer, 30(Suppl 3), A161-A161.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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