Mouse Anti-ETFA Recombinant Antibody (2A5) (V2LY-0825-LY838)

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Basic Information

Host Animal
Mouse
Clone
2A5
Application
ELISA
Immunogen
Full-length recombinant ETFA.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
electron-transfer-flavoprotein, alpha polypeptide
Entrez Gene ID
UniProt ID
Research Area
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:27499296, PubMed:15159392, PubMed:15975918, PubMed:9334218, PubMed:10356313).

It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:9334218).

Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:1882842, PubMed:1430199).
Biological Process
Fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB
Cellular Location
Mitochondrion matrix
Involvement in disease
Glutaric aciduria 2A (GA2A):
An autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
PTM
The N-terminus is blocked.

Yan, Y., Xu, Y., Yang, X., Li, Z., Niu, K., Liu, C., ... & Wu, W. (2022). Electron Transfer Flavoprotein (ETF) α Controls Blood Vessel Development by Regulating Endothelial Mitochondrial Bioenergetics and Oxygen Consumption. Oxidative medicine and cellular longevity, 2022.

Salerno, K. M., Domenico, J., Le, N. Q., Stiles, C. D., Solov’yov, I. A., & Martino, C. F. (2022). Long-Time Oxygen Localization in Electron Transfer Flavoprotein. Journal of Chemical Information and Modeling, 62(17), 4191-4199.

Park, K. H., Gooz, M., Ye, Z. W., Zhang, J., Beeson, G. C., Rockey, D. C., & Kim, S. H. (2021). Flavin Adenine Dinucleotide Depletion Caused by electron transfer flavoprotein subunit alpha Haploinsufficiency Leads to Hepatic Steatosis and Injury in Zebrafish. Hepatology communications, 5(6), 976-991.

Henriques, B. J., Olsen, R. K. J., Gomes, C. M., & Bross, P. (2021). Electron transfer flavoprotein and its role in mitochondrial energy metabolism in health and disease. Gene, 776, 145407.

Sucharitakul, J., Buckel, W., & Chaiyen, P. (2021). Rapid kinetics reveal surprising flavin chemistry in bifurcating electron transfer flavoprotein from Acidaminococcus fermentans. Journal of Biological Chemistry, 296.

Ou, M., Zhu, L., Zhang, Y., Zhang, Y., Zhou, J., Zhang, Y., ... & Wang, W. (2020). A novel electron transfer flavoprotein dehydrogenase (ETFDH) gene mutation identified in a newborn with glutaric acidemia type II: a case report of a Chinese family. BMC Medical Genetics, 21(1), 1-7.

Vogt, M. S., Schühle, K., Kölzer, S., Peschke, P., Chowdhury, N. P., Kleinsorge, D., ... & Heider, J. (2019). Structural and functional characterization of an electron transfer flavoprotein involved in toluene degradation in strictly anaerobic bacteria. Journal of Bacteriology, 201(21), e00326-19.

Yu, H. M., Yao, X. D., Zhang, R. M., Zhuang, H. F., Wang, P. W., & Li, Y. Z. (2019). Repression of let‐7b‐5p prevents the development of multifidus muscle dysfunction by promoting vitamin D accumulation via upregulation of electron transfer flavoprotein alpha subunit in a rat model of multifidus muscle injury. Journal of Cellular Biochemistry, 120(5), 7458-7473.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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