Mouse Anti-ETFB Recombinant Antibody (CBFYE-1327) (V2LY-0825-LY840)

Name: Mouse Anti-ETFB Recombinant Antibody (CBFYE-1327)
SKU: V2LY-0825-LY840
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1327

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 177-202 within an internal region of human ETFB.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Electron Transfer Flavoprotein Beta Subunit

Entrez Gene ID

2109

UniProt ID

P38117

Research Area

Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:25416781, PubMed:15159392, PubMed:15975918).

It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:7912128).

ETFB binds an AMP molecule that probably has a purely structural role (PubMed:8962055, PubMed:15159392, PubMed:15975918).

Biological Process

Fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB

Cellular Location

Mitochondrion matrix

Involvement in disease

Glutaric aciduria 2B (GA2B):
An autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.

PTM

Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from acyl-CoA dehydrogenases.

Yan, Y., Xu, Y., Yang, X., Li, Z., Niu, K., Liu, C., ... & Wu, W. (2022). Electron Transfer Flavoprotein (ETF) α Controls Blood Vessel Development by Regulating Endothelial Mitochondrial Bioenergetics and Oxygen Consumption. Oxidative medicine and cellular longevity, 2022.

Henriques, B. J., Olsen, R. K. J., Gomes, C. M., & Bross, P. (2021). Electron transfer flavoprotein and its role in mitochondrial energy metabolism in health and disease. Gene, 776, 145407.

Park, K. H., Gooz, M., Ye, Z. W., Zhang, J., Beeson, G. C., Rockey, D. C., & Kim, S. H. (2021). Flavin Adenine Dinucleotide Depletion Caused by electron transfer flavoprotein subunit alpha Haploinsufficiency Leads to Hepatic Steatosis and Injury in Zebrafish. Hepatology communications, 5(6), 976-991.

Zhang, X., Zhang, Y. L., Qiu, G., Pian, L., Guo, L., Cao, H., ... & Zhang, J. (2020). Hepatic neddylation targets and stabilizes electron transfer flavoproteins to facilitate fatty acid β-oxidation. Proceedings of the National Academy of Sciences, 117(5), 2473-2483.

Ou, M., Zhu, L., Zhang, Y., Zhang, Y., Zhou, J., Zhang, Y., ... & Wang, W. (2020). A novel electron transfer flavoprotein dehydrogenase (ETFDH) gene mutation identified in a newborn with glutaric acidemia type II: a case report of a Chinese family. BMC Medical Genetics, 21(1), 1-7.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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