Mouse Anti-ETS2 Recombinant Antibody (CBFYE-1348) (V2LY-0825-LY866)

Name: Mouse Anti-ETS2 Recombinant Antibody (CBFYE-1348)
SKU: V2LY-0825-LY866
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ETS2 Recombinant Antibody (CBFYE-1348) (V2LY-0825-LY866) in IF

Relative amounts of Ets-2 protein in the nuclei of resting and 6 h-activated naive Th cells shown by immunofluorescence. The cells were stained for the Ets-2 protein (green), and the nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI) (blue). Images are from a Leica TCSSP5 confocal microscope, 63× objective. The results shown are representative of at least three independent experiments. ...View More

Panagoulias, I., Karagiannis, F., Aggeletopoulou, I., Georgakopoulos, T., Argyropoulos, C. P., Akinosoglou, K., ... & Mouzaki, A. (2018). Ets-2 acts as a transcriptional repressor of the human immunodeficiency virus type 1 through binding to a repressor-activator target sequence of 5'-LTR. Frontiers in Immunology, 8, 1924.

Mouse Anti-ETS2 Recombinant Antibody (CBFYE-1348) (V2LY-0825-LY866) in WB

Western blot analysis to verify the silencing of Ets-2 in the transfected cells. β-Tubulin protein levels were used as an internal control for equal loading. The results shown are representative of at least three independent experiments. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1348

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 441-469 at the C-terminus of human Ets-2.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ETS Proto-Oncogene 2, Transcription Factor

Entrez Gene ID

Human	2114
Mouse	23872

UniProt ID

Human	P15036
Mouse	P15037

Research Area

Transcription factor activating transcription. Binds specifically the DNA GGAA/T core motif (Ets-binding site or EBS) in gene promoters and stimulates transcription.

Biological Process

Cell differentiation Source: GO_Central
Ectodermal cell fate commitment Source: Ensembl
Mesoderm development Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Primitive streak formation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Skeletal system development Source: ProtInc

Cellular Location

Nucleus

PTM

Phosphorylation by CDK10 at Ser-220 and Ser-225 creates a phosphodegron that targets ETS2 for proteasomal degradation.

Wang, H., Huang, R., Guo, W., Qin, X., Yang, Z., Yuan, Z., ... & Wang, H. (2020). RNA-binding protein CELF1 enhances cell migration, invasion, and chemoresistance by targeting ETS2 in colorectal cancer. Clinical Science, 134(14), 1973-1990.

Fry, E. A., & Inoue, K. (2018). Aberrant expression of ETS1 and ETS2 proteins in cancer. Cancer reports and reviews, 2(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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