Mouse Anti-ETV7 Recombinant Antibody (CBFYE-1367) (V2LY-0825-LY892)

Name: Mouse Anti-ETV7 Recombinant Antibody (CBFYE-1367)
SKU: V2LY-0825-LY892
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1367

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 111-198 mapping within an internal region of human TEL2.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ETV7

Entrez Gene ID

51513

UniProt ID

Q9Y603

Research Area

Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Isoform A does not seem to have a repressor activity. Isoform C does not seem to have a repressor activity.

Biological Process

Animal organ morphogenesis Source: ProtInc
Cell differentiation Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription by RNA polymerase II Source: ProtInc

Cellular Location

Nucleus

Froggatt, H. M., Harding, A. T., Chaparian, R. R., & Heaton, N. S. (2021). ETV7 limits antiviral gene expression and control of influenza viruses. Science signaling, 14(691), eabe1194.

Li, H., Zhang, Y., & Zheng, S. (2021). Comprehensive Analysis Identified ETV7 as a Potential Prognostic Biomarker in Bladder Cancer. BioMed research international, 2021.

Froggatt, H. M., Harding, A. T., Heaton, B. E., & Heaton, N. S. (2020). ETV7 limits antiviral gene expression and control of SARS-CoV-2 and influenza viruses. bioRxiv, 851543.

Qu, H., Zhao, H., Zhang, X., Liu, Y., Li, F., Sun, L., & Song, Z. (2020). Integrated analysis of the ETS family in melanoma reveals a regulatory role of ETV7 in the immune microenvironment. Frontiers in immunology, 11, 612784.

Alessandrini, F., Pezzè, L., Menendez, D., Resnick, M. A., & Ciribilli, Y. (2018). ETV7-mediated DNAJC15 repression leads to doxorubicin resistance in breast cancer cells. Neoplasia, 20(8), 857-870.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ETV7 Monoclonal Antibody (1A1) (CAT#: V2LY-0825-LY890)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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