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Mouse Anti-EXO1 Recombinant Antibody (1G12) (CBMAB-A2746-LY)

The product is antibody recognizes EXO1. The antibody 1G12 immunoassay techniques such as: WB, ELISA.
See all EXO1 antibodies
Published Data
Fig.1 Western blot showing the expression of EXO1 in a panel of breast cancer cell lines.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1G12
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EXO1 (AAH07491, 747 a.a. ~ 846 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Exonuclease 1
Introduction
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]
Entrez Gene ID
9156
UniProt ID
Q9UQ84
Alternative Names
HEX1; hExoI
Research Area
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Biological Process
DNA recombination Source: UniProtKB
DNA repair Source: ProtInc
Humoral immune response mediated by circulating immunoglobulin Source: Ensembl
Isotype switching Source: Ensembl
Meiotic cell cycle Source: UniProtKB-KW
Mismatch repair Source: UniProtKB
Somatic hypermutation of immunoglobulin genes Source: Ensembl
T-circle formation Source: BHF-UCL
Cellular Location
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
PTM
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilization/degradation of the protein.

Kadyrova, L. Y., Dahal, B. K., Gujar, V., Daley, J. M., Sung, P., & Kadyrov, F. A. (2022). The nuclease activity of DNA2 promotes exonuclease 1–independent mismatch repair. Journal of Biological Chemistry, 298(4).

Mao, P., Wu, S., & Fan, Y. (2022). Upregulation of exonuclease 1 caused by homology-dependent repair confers cisplatin resistance to gastric cancer cells. Canadian Journal of Physiology and Pharmacology, 100(9), 903-914.

Yan, S., Gao, S., & Zhou, P. (2021). Multi-functions of exonuclease 1 in DNA damage response and cancer susceptibility. Radiation Medicine and Protection, 2(4), 146-154.

Zhou, C. S., Feng, M. T., Chen, X., Gao, Y., Chen, L., Li, L. D., ... & Cao, Y. Q. (2021). Exonuclease 1 (EXO1) is a potential prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma. OncoTargets and therapy, 14, 1033.

Kratz, K., Artola-Borán, M., Kobayashi-Era, S., Koh, G., Oliveira, G., Kobayashi, S., ... & Jiricny, J. (2021). FANCD2-associated nuclease 1 partially compensates for the lack of Exonuclease 1 in mismatch repair. Molecular and Cellular Biology, 41(9), e00303-21.

He, D., Li, T., Sheng, M., & Yang, B. (2020). Exonuclease 1 (Exo1) participates in mammalian non-homologous end joining and contributes to drug resistance in ovarian cancer. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 26, e918751-1.

Luo, F., Wang, Y. Z., Lin, D., Li, J., & Yang, K. (2019). Exonuclease 1 expression is associated with clinical progression, metastasis, and survival prognosis of prostate cancer. Journal of Cellular Biochemistry, 120(7), 11383-11389.

Wang, W., Daley, J. M., Kwon, Y., Xue, X., Krasner, D. S., Miller, A. S., ... & Sung, P. (2018). A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1–Dna2 in long-range DNA end resection. Journal of Biological Chemistry, 293(44), 17061-17069.

Keijzers, G., Bakula, D., Petr, M. A., Madsen, N. G. K., Teklu, A., Mkrtchyan, G., ... & Scheibye-Knudsen, M. (2018). Human exonuclease 1 (EXO1) regulatory functions in DNA replication with putative roles in cancer. International journal of molecular sciences, 20(1), 74.

Yan, J., Shun, M. C., Hao, C., Zhang, Y., Qian, J., Hrecka, K., ... & Skowronski, J. (2018). HIV-1 Vpr reprograms CLR4DCAF1 E3 ubiquitin ligase to antagonize exonuclease 1-mediated restriction of HIV-1 infection. MBio, 9(5), e01732-18.

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For research use only. Not intended for any clinical use.

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