Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952)

Name: Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395)
SKU: V2LY-0825-LY952
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952) in IF

Co-localization of Flag-USP36 and EXOSC10. HeLa cells transfected with Flag-USP36 were immunostained with anti-Flag (red) and anti-EXOSC10 (green). The nuclei were stained with DAPI (blue). ...View More

Chen, Y., Li, Y., Dai, R. S., Savage, J. C., Shinde, U., Klimek, J., ... & Dai, M. S. (2023). The ubiquitin-specific protease USP36 SUMOylates EXOSC10 and promotes the nucleolar RNA exosome function in rRNA processing. Nucleic acids research, 51(8), 3934-3949.

Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952) in IF

Depletion of EXOSC10 impairs RPA and RAD51 recruitment to DSBs. a The panel shows RAD51 immunofluorescent staining of HeLa cells depleted of either EXOSC10 or DIS3 for 48 h. The cells were fixed 30 min after UV laser micro-irradiation. The bar plot in the lower part of the figure shows the percentage of γH2AX-positive stripes that were co-stained by RAD51 (n > 35 cells analysed in each condition, from two independent experiments). b RPA immunofluorescent staining was performed on cells depleted of either EXOSC10 or DIS3 and fixed 15 min after UV micro-irradiation. The bar plot shows the percentage of γH2AX-positive stripes that were co-stained by RPA (n > 60 cells analysed for each siRNA treatment, from at least two independent experiments). c CtIP immunofluorescent staining was performed on cells depleted of either EXOSC10 or DIS3 and fixed 10 min after UV micro-irradiation. ...View More

Domingo-Prim, J., Endara-Coll, M., Bonath, F., Jimeno, S., Prados-Carvajal, R., Friedländer, M. R., ... & Visa, N. (2019). EXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks. Nature communications, 10(1), 2135.

Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952) in WB

Western blot of extracts derived from HCT116 cells transfected with control or WDR82-specific siRNAs. The blot shows WDR82 and, as a loading control, EXOSC10. ...View More

Estell, C., Davidson, L., Steketee, P. C., Monier, A., & West, S. (2021). ZC3H4 restricts non-coding transcription in human cells. Elife, 10, e67305.

Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952) in IHC

Confocal microscopy after MIRLET7D-FISH and EXOSC10 immunostaining shows nuclear colocalization of MIRLET7D and EXOSC10 in Ctrl primary human lung fibroblasts and reduced level of MIRLET7D in IPF primary human lung fibroblasts. DAPI, nucleus. Scale bars, 5 μm. Squares are respectively shown below at higher magnification. ...View More

Rubio, K., Singh, I., Dobersch, S., Sarvari, P., Günther, S., Cordero, J., ... & Barreto, G. (2019). Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nature communications, 10(1), 2229.

Mouse Anti-EXOSC10 Recombinant Antibody (CBFYE-1395) (V2LY-0825-LY952) in WB

Western blotting of EXOSC10 in either parental Tir1-expressing HCT116 (HCT116:TIR1) or EXOSC10-AID cells. A time course of auxin addition was applied to the EXOSC10-AID cells. Equal loading is shown by the presence of a non-specific product (∗) on the same blot. ...View More

Davidson, L., Francis, L., Cordiner, R. A., Eaton, J. D., Estell, C., Macias, S., ... & West, S. (2019). Rapid depletion of DIS3, EXOSC10, or XRN2 reveals the immediate impact of exoribonucleolysis on nuclear RNA metabolism and transcriptional control. Cell reports, 26(10), 2779-2791.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1395

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-300 mapping at the N-terminus of human EXOSC10.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

exosome component 10

Entrez Gene ID

Human	5394
Mouse	50912
Rat	313707

UniProt ID

Human	Q01780
Mouse	P56960
Rat	D4A1X2

Research Area

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity).

EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPHOSPH6 with the RNA exosome involved in the maturation of 5.8S rRNA.

Biological Process

CUT catabolic process Source: UniProtKB
Dosage compensation by inactivation of X chromosome Source: Ensembl
Exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
Histone mRNA catabolic process Source: UniProtKB
Maturation of 5.8S rRNA Source: UniProtKB
Negative regulation of telomere maintenance via telomerase Source: BHF-UCL
Nuclear mRNA surveillance Source: UniProtKB
Nuclear polyadenylation-dependent antisense transcript catabolic process Source: GO_Central
Nuclear polyadenylation-dependent CUT catabolic process Source: GO_Central
Nuclear polyadenylation-dependent rRNA catabolic process Source: UniProtKB
Nuclear polyadenylation-dependent snoRNA catabolic process Source: GO_Central
Nuclear polyadenylation-dependent snRNA catabolic process Source: GO_Central
Nuclear polyadenylation-dependent tRNA catabolic process Source: GO_Central
Nuclear retention of unspliced pre-mRNA at the site of transcription Source: UniProtKB
Nuclear-transcribed mRNA catabolic process Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Polyadenylation-dependent snoRNA 3'-end processing Source: GO_Central
Regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
rRNA processing Source: Reactome

Cellular Location

Nucleolus; Nucleus; Cytoplasm. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.

Petit, F. G., Jamin, S. P., Kernanec, P. Y., Becker, E., Halet, G., & Primig, M. (2022). EXOSC10/Rrp6 is essential for the eight-cell embryo/morula transition. Developmental Biology, 483, 58-65.

Stuparević, I., Novačić, A., Rahmouni, A. R., Fernandez, A., Lamb, N., & Primig, M. (2021). Regulation of the conserved 3′‐5′ exoribonuclease EXOSC10/Rrp6 during cell division, development and cancer. Biological Reviews, 96(4), 1092-1113.

Stuparevic, I., Novacic, A., Oskomic, M., Strbac, L., Beauvais, V., Primig, M., & Rahmouni, A. R. (2021). Roles of Rrp6/EXOSC10-targeted lncRNAs in anti-cancer drug toxicity and cell wall architecture. FEBS Open Bio, 11, 102-102.

Wu, D., & Dean, J. (2020). EXOSC10 sculpts the transcriptome during the growth-to-maturation transition in mouse oocytes. Nucleic acids research, 48(10), 5349-5365.

Oreški, L. (2019). Analiza ekspresije exosc10/Exosc10 u tkivima zebrice (Danio rerio) i embrionalnim fazama razvoja (Doctoral dissertation, University of Zagreb. Faculty of Food Technology and Biotechnology. Department of Chemistry and Biochemistry. Laboratory for Biochemistry).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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