Mouse Anti-EYA3 Recombinant Antibody (CBFYE-1426) (V2LY-0825-LY990)

Name: Mouse Anti-EYA3 Recombinant Antibody (CBFYE-1426)
SKU: V2LY-0825-LY990
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1426

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 207-300 mapping within an internal region of human EYA3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Eya Transcriptional Coactivator And Phosphatase 3

Entrez Gene ID

2140

UniProt ID

Q99504

Research Area

Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1 (PubMed:19234442, PubMed:19351884).

Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity).

May be involved in development of the eye.

Biological Process

Anatomical structure development Source: GO_Central
Anatomical structure morphogenesis Source: ProtInc
Cell differentiation Source: GO_Central
Double-strand break repair Source: UniProtKB
Histone dephosphorylation Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Positive regulation of DNA repair Source: UniProtKB
Response to ionizing radiation Source: UniProtKB
Visual perception Source: ProtInc

Cellular Location

Nucleus; Cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:19234442). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively (By similarity).

PTM

Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.

Yang, C., & Liu, H. (2022). Both a hypoxia-inducible EYA3 and a histone acetyltransferase p300 function as coactivators of SIX5 to mediate tumorigenesis and cancer progression. Annals of translational medicine, 10(13).

Xu, S., Lian, Z., Zhang, S., Xu, Y., & Zhang, H. (2021). CircGNG4 promotes the progression of prostate cancer by sponging miR-223 to enhance EYA3/c-myc expression. Frontiers in Cell and Developmental Biology, 9.

Ionescu, A. E., Mentel, M., Munteanu, C. V., Sima, L. E., Martin, E. C., Necula-Petrareanu, G., & Szedlacsek, S. E. (2019). Analysis of EYA3 phosphorylation by Src kinase identifies residues involved in cell proliferation. International journal of molecular sciences, 20(24), 6307.

Wang, Y., Pandey, R. N., York, A. J., Mallela, J., Nichols, W. C., Hu, Y. C., ... & Hegde, R. S. (2019). The EYA3 tyrosine phosphatase activity promotes pulmonary vascular remodeling in pulmonary arterial hypertension. Nature communications, 10(1), 1-13.

Vartuli, R. L., Zhou, H., Zhang, L., Powers, R. K., Klarquist, J., Rudra, P., ... & Ford, H. L. (2018). Eya3 promotes breast tumor–associated immune suppression via threonine phosphatase–mediated PD-L1 upregulation. The Journal of clinical investigation, 128(6), 2535-2550.

Zhang, L., Zhou, H., Li, X., Vartuli, R. L., Rowse, M., Xing, Y., ... & Ford, H. L. (2018). Eya3 partners with PP2A to induce c-Myc stabilization and tumor progression. Nature communications, 9(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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