Rabbit Anti-F8 Recombinant Antibody (CBYY-C2452) (V2LY-0825-LY1161)

Name: Rabbit Anti-F8 Recombinant Antibody (CBYY-C2452)
SKU: V2LY-0825-LY1161
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-C2452

Application

ELISA

Immunogen

Recombinant Human Coagulation Factor VIII / FVIII / F8 protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:5,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Coagulation Factor VIII

Entrez Gene ID

2157

UniProt ID

P00451

Research Area

Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.

Biological Process

Acute-phase response Source: UniProtKB-KW
Blood coagulation Source: ProtInc
Blood coagulation, intrinsic pathway Source: GO_Central

Cellular Location

Extracellular space

Involvement in disease

Hemophilia A (HEMA):
The disease is caused by variants affecting the gene represented in this entry. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery.

PTM

Sulfation on Tyr-1699 is essential for binding vWF.

Han, J. P., Song, D. W., Lee, J. H., Lee, G. S., & Yeom, S. C. (2021). Novel severe hemophilia a mouse model with factor VIII intron 22 inversion. Biology, 10(8), 704.

Collins, A. E., Curry, N., Raza‐Burton, S., Bignell, P., Davies, C., Joffe, M., ... & Desborough, M. J. (2021). Factor VIII levels and bleeding according to factor 8 (F8) mutation in pregnant carriers of haemophilia A: a multicentre retrospective cohort study. British journal of haematology, 193(2), 397-400.

Simioni, P., Cagnin, S., Sartorello, F., Sales, G., Pagani, L., Bulato, C., ... & Castoldi, E. (2021). Partial F8 gene duplication (factor VIII Padua) associated with high factor VIII levels and familial thrombophilia. Blood, 137(17), 2383-2393.

Gong, J., Chung, T. H., Zheng, J., Zheng, H., & Chang, L. J. (2021). Transduction of modified factor VIII gene improves lentiviral gene therapy efficacy for hemophilia A. Journal of Biological Chemistry, 297(6).

Mamoor, S. (2021). Differential expression of coagulation factor VIII in cancers of the breast.

Chen, C. Y., Tran, D. M., Cavedon, A., Cai, X., Rajendran, R., Lyle, M. J., ... & Miao, C. H. (2020). Treatment of hemophilia A using factor VIII messenger RNA lipid nanoparticles. Molecular Therapy-Nucleic Acids, 20, 534-544.

Chen, H., Shi, M., Gilam, A., Zheng, Q., Zhang, Y., Afrikanova, I., ... & Chen-Tsai, R. Y. (2019). Hemophilia A ameliorated in mice by CRISPR-based in vivo genome editing of human Factor VIII. Scientific reports, 9(1), 1-15.

Neumeyer, J., Lin, R. Z., Wang, K., Hong, X., Hua, T., Croteau, S. E., ... & Melero-Martin, J. M. (2019). Bioengineering hemophilia A–specific microvascular grafts for delivery of full-length factor VIII into the bloodstream. Blood advances, 3(24), 4166-4176.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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