Rat Anti-FABP3 Recombinant Antibody (CBXF-2299) (CBMAB-F1612-CQ)

Name: Rat Anti-FABP3 Recombinant Antibody (CBXF-2299)
SKU: CBMAB-F1612-CQ
Rating: 5 (1 reviews)

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Published Data

Rat Anti-FABP3 Recombinant Antibody (CBXF-2299) (CBMAB-F1612-CQ) in WB

Recombinant human FABP3 expression, production, and purification: (A) Western Blot analysis of FABP3 expression after the isopropylthiogalactoside (IPTG) induction. Lanes 1 and 5 correspond to non-induced bacterial cultures, while lanes 2, 3, 4, 6, and 7 correspond to the IPTG-treated bacterial cultures. (B) Purification of N-terminal His-tagged FABP3 by immobilized metal affinity chromatography (IMAC): Lanes 1, 2, and 3 show the total cell extract, cell pellet, and the soluble extract, respectively. Lanes 4, 5, 6, 7, and 8 show fractionated eluents. (C) Consecutive purification of N-terminal His-tagged FABP3 by size exclusion chromatography (SEC). (D) Chromatogram shows two peaks: the blue peak (UV280 nm) corresponds to the purified His-tagged FABP3, and the yellow peak (conductivity) corresponds to impurities in the both Bradford protein assay and Coomassie Blue stain. FABP3 concentration was quantified after purification with a BioSpec nano spectrophotometer using the extinction coefficient (Ɛ) = 18,450 M−1 cm−1 and molecular mass = 17,592.03 g/mol at 280 nm. Quantitation showed a total yield of 10 mg/mL purified FABP3 after three consecutive runs of size exclusion chromatography. ...View More

Ayo, A., Figueras, E., Schachtsiek, T., Budak, M., Sewald, N., & Laakkonen, P. (2020). Tumor-targeting peptides: the functional screen of glioblastoma homing peptides to the target protein FABP3 (MDGI). Cancers, 12(7), 1836.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

CBXF-2299

Application

WB, IHC

Immunogen

E. coli-derived recombinant human FABP3/H‑FABP (Met1-Ala133).

Host Species

Rat

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.5 μg/ml
IHC	8-25 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fatty Acid Binding Protein 3

Introduction

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	2170
Mouse	14077
Rat	79131

UniProt ID

Human	P05413
Mouse	P11404
Rat	P07483

Alternative Names

Fatty Acid Binding Protein 3; Mammary-Derived Growth Inhibitor; Fatty Acid Binding Protein 3, Muscle And Heart; Heart-Type Fatty Acid-Binding Protein; Muscle Fatty Acid-Binding Protein; Fatty Acid Binding Protein 11; FABP11; H-FABP;

Research Area

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.

Biological Process

Brown fat cell differentiation Source: Ensembl
Cholesterol homeostasis Source: BHF-UCL
Fatty acid metabolic process Source: Ensembl
Intracellular lipid transport Source: BHF-UCL
Long-chain fatty acid transport Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ProtInc
Phospholipid homeostasis Source: BHF-UCL
Positive regulation of long-chain fatty acid import into cell Source: ARUK-UCL
Positive regulation of phospholipid biosynthetic process Source: BHF-UCL
Regulation of fatty acid oxidation Source: BHF-UCL
Regulation of phosphatidylcholine biosynthetic process Source: Ensembl
Response to drug Source: Ensembl
Response to fatty acid Source: Ensembl
Response to insulin Source: Ensembl

Cellular Location

Cytoplasm

Dogra, A., Kour, D., Gour, A., Bhardwaj, M., Bag, S., Dhiman, S. K., ... & Nandi, U. (2022). Ameliorating effect of rutin against diclofenac-induced cardiac injury in rats with underlying function of FABP3, MYL3, and ANP. Drug and Chemical Toxicology, 1-12.

Liu, Z. Z., Hong, C. G., Hu, W. B., Chen, M. L., Duan, R., Li, H. M., ... & Xie, H. (2021). Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy, 17(10), 2766-2782.

Matsuo, K., Kawahata, I., Melki, R., Bousset, L., Owada, Y., & Fukunaga, K. (2021). Suppression of α-synuclein propagation after intrastriatal injection in FABP3 null mice. Brain research, 1760, 147383.

Lee, S. M., Lee, S. H., Jung, Y., Lee, Y., Yoon, J. H., Choi, J. Y., ... & Kwon, K. S. (2020). FABP3-mediated membrane lipid saturation alters fluidity and induces ER stress in skeletal muscle with aging. Nature communications, 11(1), 1-15.

Ayo, A., Figueras, E., Schachtsiek, T., Budak, M., Sewald, N., & Laakkonen, P. (2020). Tumor-Targeting Peptides: The Functional Screen of Glioblastoma Homing Peptides to the Target Protein FABP3 (MDGI). Cancers, 12(7), 1836.

Bi, Y., Yuan, X., Zhu, P., Chen, Y., Chen, G., & Chang, G. (2020). A novel long noncoding RNA, ENSGALG00000021686, regulates the intracellular transport of fatty acids by targeting the FABP3 gene in chicken. Biochemical and Biophysical Research Communications, 528(4), 706-712.

Cheng, A., Shinoda, Y., Yamamoto, T., Miyachi, H., & Fukunaga, K. (2019). Development of FABP3 ligands that inhibit arachidonic acid-induced α-synuclein oligomerization. Brain research, 1707, 190-197.

Yamamoto, Y., Kida, H., Kagawa, Y., Yasumoto, Y., Miyazaki, H., Islam, A., ... & Owada, Y. (2018). FABP3 in the anterior cingulate cortex modulates the methylation status of the glutamic acid decarboxylase67 promoter region. Journal of Neuroscience, 38(49), 10411-10423.

Sepe, F. N., Chiasserini, D., & Parnetti, L. (2018). Role of FABP3 as biomarker in Alzheimer's disease and synucleinopathies. Future Neurology, 13(4), 199-207.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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