Mouse Anti-FAF2 Recombinant Antibody (CBCNC-599) (CBMAB-C3445-CN)

Name: Mouse Anti-FAF2 Recombinant Antibody (CBCNC-599)
SKU: CBMAB-C3445-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FAF2 Recombinant Antibody (CBCNC-599) (CBMAB-C3445-CN) in WB

WB analysis of the pull-down of VCP in cells treated as in (D). The levels of VCP, SUMO2/3, FAF1, and CDK1 were analyzed with specific antibodies. 5% of the input material is shown (Input). A control immunoprecipitation with a non-specific IgG was performed (IgG) and compared to the pull-down of VCP (VCP). ...View More

Franz, A., Valledor, P., Ubieto-Capella, P., Pilger, D., Galarreta, A., Lafarga, V., ... & Lecona, E. (2021). USP7 and VCPFAF1 define the SUMO/Ubiquitin landscape at the DNA replication fork. Cell Reports, 37(2).

Mouse Anti-FAF2 Recombinant Antibody (CBCNC-599) (CBMAB-C3445-CN) in WB

In vitro assays to test ubiquitination of E6AP substrates identified by OUT. wt UB was transferred through wt Uba1-UbcH7-E6AP cascade to the potential substrate proteins expressed from E. coli cells. E6AP ubiquitination of MAPK1 a, PRMT5 b, CDK1 c, CDK4 d, β-catenin e, and UbxD8 f, were confirmed. E6AP-catalyzed ubiquitination of HHR23A, a previously reported E6AP substrate, was also assayed g. All blots are representative of at least three independent experiments. ...View More

Wang, Y., Liu, X., Zhou, L., Duong, D., Bhuripanyo, K., Zhao, B., ... & Yin, J. (2017). Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer. Nature communications, 8(1), 2232.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBCNC-599

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 147-434 mapping at the C-terminus of human ETEA.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fas Associated Factor Family Member 2

Introduction

The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD), compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	23197
Mouse	76577
Rat	291000

UniProt ID

Human	Q96CS3
Mouse	Q3TDN2
Rat	Q5BK32

Alternative Names

Fas Associated Factor Family Member 2; UBX Domain-Containing Protein 3B; UBX Domain-Containing Protein 8; UBX Domain Containing 8; UBX Domain Protein 3B; UBXN3B; UBXD8;

Research Area

Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:18711132, PubMed:24215460).

By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed:26692333).

Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis (PubMed:23297223).

Biological Process

Lipid droplet organization Source: MGI
Response to unfolded protein Source: UniProtKB-KW
Retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Ubiquitin-dependent ERAD pathway Source: UniProtKB

Cellular Location

Endoplasmic reticulum; Cytoplasm; Lipid droplet

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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