Mouse Anti-FAM49B Recombinant Antibody (F2993) (V2LY-0825-LY1345)

Name: Mouse Anti-FAM49B Recombinant Antibody (F2993)
SKU: V2LY-0825-LY1345
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FAM49B Recombinant Antibody (F2993) (V2LY-0825-LY1345) in WB

Expression of the indicated proteins in control and mutant platelets was assessed by Western blot analysis with α-Tubulin serving as loading control (n = 3). ...View More

Sisario, D., Spindler, M., Ermer, K. J., Grütz, N., Nicolai, L., Gaertner, F., ... & Bender, M. (2024). Differential Role of the RAC1-Binding Proteins FAM49b (CYRI-B) and CYFIP1 in Platelets. Cells, 13(4), 299.

Mouse Anti-FAM49B Recombinant Antibody (F2993) (V2LY-0825-LY1345) in WB

The protein expression levels of EMT markers in control group and CYRI-B knockdown group after being treated with stat3 inhibitor HO-3867 detected by Western blot. ...View More

Liu, G., Xia, Y., Wang, H., Jin, X., Chen, S., Chen, W., ... & He, Y. (2023). Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer. Experimental cell research, 423(1), 113453.

Mouse Anti-FAM49B Recombinant Antibody (F2993) (V2LY-0825-LY1345) in IF

The protein levels of EMT marker and CYRI-B in CYRI-B knock-down and control group by Immunofluorescence. ...View More

Mouse Anti-FAM49B Recombinant Antibody (F2993) (V2LY-0825-LY1345) in IHC

Representative images of immunohistochemistry staining of GC tissues and adjacent paracarcinoma tissues for CYRI-B. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

F2993

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 8-20 near the N-terminus of human FAM49B.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

family with sequence similarity 49, member B

Entrez Gene ID

51571

UniProt ID

Q9NUQ9

Research Area

Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:31285585, PubMed:30250061).

Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (PubMed:30250061).

Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585).

Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (By similarity).

Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).

Biological Process

Cellular response to molecule of bacterial origin Source: UniProtKB
Negative regulation of actin filament polymerization Source: UniProtKB
Negative regulation of small GTPase mediated signal transduction Source: UniProtKB
Positive regulation of interferon-gamma production Source: UniProtKB
Positive regulation of memory T cell activation Source: UniProtKB
Positive regulation of T cell activation Source: UniProtKB
Positive regulation of T cell mediated cytotoxicity Source: UniProtKB
Regulation of cell migration Source: UniProtKB
Regulation of chemotaxis Source: UniProtKB
Regulation of establishment of cell polarity Source: UniProtKB
Regulation of mitochondrial fission Source: UniProtKB

Cellular Location

Mitochondrion; Membrane

PTM

Ubiquitinated at Lys-74 upon Salmonella bacterial infection.

Huang, Z., Pu, J., Luo, Y., Fan, J., Li, K., Peng, D., ... & Zhou, F. (2022). FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner. Molecular Immunology, 143, 135-146.

Chen, Y., Jiang, Y., Lao, J., Zhou, Y., Su, L., & Huang, X. (2022). Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells. Genes, 13(2), 388.

Xu, F., Chen, J., & Huang, D. (2022). Pan-cancer analysis identifies FAM49B as an immune-related prognostic maker for hepatocellular carcinoma. Journal of Cancer, 13(1), 278.

Park, C. S., Guan, J., Rhee, P., Gonzalez, F., Coscoy, L., Robey, E. A., ... & Sadegh-Nasseri, S. (2022). Fam49b dampens TCR signal strength to regulate survival of positively selected thymocytes. bioRxiv.

Li, Y., Xiong, Y., Wang, Z., Han, J., Shi, S., He, J., ... & Liu, W. (2021). FAM49B promotes breast cancer proliferation, metastasis, and chemoresistance by stabilizing ELAVL1 protein and regulating downstream Rab10/TLR4 pathway. Cancer Cell International, 21(1), 1-20.

Zhang, Y., Du, P., Li, Y., Zhu, Q., Song, X., Liu, S., ... & Liu, Y. (2020). TASP1 promotes gallbladder cancer cell proliferation and metastasis by up-regulating FAM49B via PI3K/AKT pathway. International journal of biological sciences, 16(5), 739.

Li, W. R., Guan, X. L., Jiang, S., & Sun, L. (2020). The novel fish miRNA pol-miR-novel_171 and its target gene FAM49B play a critical role in apoptosis and bacterial infection. Developmental & Comparative Immunology, 106, 103616.

Kaplan, E., Stone, R., Hume, P. J., Greene, N. P., & Koronakis, V. (2020). Structure of CYRI-B (FAM49B), a key regulator of cellular actin assembly. Acta Crystallographica Section D: Structural Biology, 76(10), 1015-1024.

Chattaragada, M. S., Riganti, C., Sassoe, M., Principe, M., Santamorena, M. M., Roux, C., ... & Novelli, F. (2018). FAM49B, a novel regulator of mitochondrial function and integrity that suppresses tumor metastasis. Oncogene, 37(6), 697-709.

Shang, W., Jiang, Y., Boettcher, M., Ding, K., Mollenauer, M., Liu, Z., ... & Wang, H. (2018). Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation. Proceedings of the National Academy of Sciences, 115(17), E4051-E4060.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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