Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698)

Name: Mouse Anti-FASLG Recombinant Antibody (CBT3829)
SKU: V2LY-0625-LY1698
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698) in ELISA

Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698) in WB

Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698) in IF

Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698) in WB

Mouse Anti-FASLG Recombinant Antibody (CBT3829) (V2LY-0625-LY1698) in FC

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3829

Application

ICC, FC

Immunogen

Purified recombinant fragment of human CD178 (AA: extra 103-281) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:50-1:200
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fas Ligand

Entrez Gene ID

356

UniProt ID

P48023

Alternative Names

Fas Ligand

Research Area

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058).

Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603).

Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity).

TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity).

Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).

Tumor necrosis factor ligand superfamily member 6, soluble form:
Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity).

Can induce apoptosis but does not appear to be essential for this process (PubMed:27806260).

FasL intracellular domain:
Cytoplasmic form induces gene transcription inhibition.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: BHF-UCL
Apoptotic process Source: ProtInc
Apoptotic signaling pathway Source: BHF-UCL
Cell-cell signaling Source: ProtInc
Cellular chloride ion homeostasis Source: Ensembl
Cellular response to interferon-gamma Source: Ensembl
Endosomal lumen acidification Source: Ensembl
Extrinsic apoptotic signaling pathway Source: UniProtKB
Extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
Inflammatory cell apoptotic process Source: Ensembl
Necroptotic process Source: BHF-UCL
Necroptotic signaling pathway Source: BHF-UCL
Negative regulation of angiogenesis Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of endothelial cell apoptotic process Source: BHF-UCL
Positive regulation of epidermal growth factor receptor signaling pathway Source: Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of neuron apoptotic process Source: Ensembl
Positive regulation of phosphatidylserine exposure on apoptotic cell surface Source: UniProtKB
Release of sequestered calcium ion into cytosol by endoplasmic reticulum Source: MGI
Response to growth factor Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Retinal cell programmed cell death Source: Ensembl
Signal transduction Source: ProtInc
T cell apoptotic process Source: UniProtKB

Cellular Location

Lysosome lumen; Cell membrane; Cytoplasmic vesicle lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (PubMed:17164290). Colocalizes with the SPPL2A protease at the cell membrane (PubMed:17557115).
Tumor necrosis factor ligand superfamily member 6, soluble form: Secreted. May be released into the extracellular fluid by cleavage from the cell surface.
FasL intracellular domain: Nucleus. The FasL ICD cytoplasmic form is translocated into the nucleus.

Involvement in disease

Autoimmune lymphoproliferative syndrome 1B (ALPS1B):
A disorder of apoptosis that manifests in early childhood and results in the accumulation of autoreactive lymphocytes. It is characterized by non-malignant lymphadenopathy with hepatosplenomegaly, and autoimmune hemolytic anemia, thrombocytopenia and neutropenia.

Topology

Cytoplasmic: 1-80
Helical: 81-102
Extracellular: 103-281

PTM

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.
N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.
Monoubiquitinated.

Wang, G., Duan, P., Liu, F., & Wei, Z. (2021). Long non-coding RNA CASC7 suppresses malignant behaviors of breast cancer by regulating miR-21-5p/FASLG axis. Bioengineered, 12(2), 11555-11566.

Ji, W., Xin, Y., Zhang, L., & Liu, X. (2020). ALG2 Influences T cell apoptosis by regulating FASLG intracellular transportation. Biochemical Journal, 477(16), 3105-3121.

Zou, L., Ma, X., Wu, B., Chen, Y., Xie, D., & Peng, C. (2020). Protective effect of bone marrow mesenchymal stem cell-derived exosomes on cardiomyoblast hypoxia-reperfusion injury through the miR-149/let-7c/Faslg axis. Free Radical Research, 54(10), 722-731.

Pang, T., Du, L., Li, F., Liu, Y., Ma, X., Cao, Q., ... & Yang, P. (2020). Association of apoptosis genes in PDCD1 but not PDCD1LG2, FAS, and FASLG with pediatric idiopathic uveitis in Han Chinese. Pediatric Research, 87(4), 634-638.

de Almeida Chuffa, L. G., dos Santos Souza, J., de Mello, M. C., de Oliveira Neto, M., & Carvalho, R. F. (2020). The aging whole blood transcriptome reveals a potential role of FASLG in COVID-19. bioRxiv.

Tang, M., Pan, H., Zheng, Z., Guo, Y., Peng, J., Yang, J., ... & Zhou, Y. (2019). Prostaglandin E1 protects cardiomyocytes against hypoxia-reperfusion induced injury via the miR-21-5p/FASLG axis. Bioscience reports, 39(12).

Du, X., Hong, L., Sun, L., Sang, H., Qian, A., Li, W., ... & Li, X. (2019). miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis. Journal of translational medicine, 17(1), 1-13.

Chen, S., Yang, C., Sun, C., Sun, Y., Yang, Z., Cheng, S., & Zhuge, B. (2019). miR-21-5p suppressed the sensitivity of hepatocellular carcinoma cells to cisplatin by targeting FASLG. DNA and cell biology, 38(8), 865-873.

Li, Y., Sun, Y., Cai, M., Zhang, H., Gao, N., Huang, H., ... & Yao, D. (2018). Fas Ligand Gene (Faslg) plays an important role in nerve degeneration and regeneration after rat sciatic nerve injury. Frontiers in Molecular Neuroscience, 11, 210.

Blaes, J., Thomé, C. M., Pfenning, P. N., Rübmann, P., Sahm, F., Wick, A., ... & Lemke, D. (2018). Inhibition of CD95/CD95L (FAS/FASLG) Signaling with APG101 Prevents Invasion and Enhances Radiation Therapy for GlioblastomaInhibition of CD95/CD95L Signaling by APG101. Molecular Cancer Research, 16(5), 767-776.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

FASLG Matched Antibody Pair (411) (CAT#: APMAB-411LY)

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For research use only. Not intended for any clinical use.

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