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Mouse Anti-FIS1 Recombinant Antibody (CBXF-2173) (V2LY-0925-LY1986)

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Published Data
V2LY-0925-LY1986-1
Mouse Anti-FIS1 Recombinant Antibody (CBXF-2173) (V2LY-0925-LY1986) in WB
(A-G) Effect on mitochondrial dynamics and apoptosis: (A-C) changes in levels of mitochondrial fission markers, such as Fis1 and Drp1 and the fusion marker MFN2. Representative western blots and densitometric quantification of (A) Fis1, (B) Drp1, and (C) MFN2. (D-G) Impact on apoptosis-related markers BID, Bax, Bcl-2, and cleaved caspase 3. Representative western blots and densitometric quantification of (D) BID, (E) Bax, (F) Bcl-2, and (G) cleaved caspase 3. ...View More
Lee, J. M., Hwang, J. W., Kim, M. J., Jung, S. Y., Kim, K. S., Ahn, E. H., ... & Choi, Y. S. (2021). Mitochondrial transplantation modulates inflammation and apoptosis, alleviating tendinopathy both in vivo and in vitro. Antioxidants, 10(5), 696.
V2LY-0925-LY1986-2
Mouse Anti-FIS1 Recombinant Antibody (CBXF-2173) (V2LY-0925-LY1986) in WB
(A-C): changes in levels of mitochondrial fission markers such as Fis1 and Drp1 and the fusion marker MFN2. Representative western blots and densitometric quantification of (A) Fis1, (B) Drp1, and (C) MFN2. ...View More
Lee, J. M., Hwang, J. W., Kim, M. J., Jung, S. Y., Kim, K. S., Ahn, E. H., ... & Choi, Y. S. (2021). Mitochondrial transplantation modulates inflammation and apoptosis, alleviating tendinopathy both in vivo and in vitro. Antioxidants, 10(5), 696.
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBXF-2173
Application
WB, IP, IF, ELISA
Immunogen
Full length human FIS1 Recombinant protein.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Entrez Gene ID
51024
UniProt ID
Q9Y3D6
Function
Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
Biological Process
Autophagy of mitochondrion Source: UniProtKB
Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
Cellular response to glucose stimulus Source: Ensembl
Cellular response to peptide Source: Ensembl
Cellular response to thapsigargin Source: Ensembl
Cellular response to toxic substance Source: Ensembl
Mitochondrial fission Source: UniProtKB
Mitochondrial fragmentation involved in apoptotic process Source: UniProtKB
Mitochondrial fusion Source: UniProtKB
Mitochondrion morphogenesis Source: UniProtKB
Negative regulation of ATP metabolic process Source: ARUK-UCL
Negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
Negative regulation of fatty acid transport Source: ARUK-UCL
Peroxisome fission Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
Positive regulation of mitochondrial fission Source: UniProtKB
Positive regulation of neuron apoptotic process Source: Ensembl
Positive regulation of protein targeting to membrane Source: UniProtKB
Protein targeting to mitochondrion Source: UniProtKB
Regulation of mitochondrion organization Source: UniProtKB
Release of cytochrome c from mitochondria Source: UniProtKB
Response to flavonoid Source: Ensembl
Response to fluoride Source: Ensembl
Response to hypobaric hypoxia Source: Ensembl
Response to muscle activity Source: Ensembl
Response to nutrient levels Source: Ensembl
Cellular Location
Peroxisome membrane; Mitochondrion outer membrane
Topology
Cytoplasmic: 1-122
Helical: 123-143
Lumenal: 144-152
PTM
Ubiquitinated by MARCHF5.

Schrader, T. A., Carmichael, R. E., Islinger, M., Costello, J. L., Hacker, C., Bonekamp, N. A., ... & Schrader, M. (2022). PEX11β and FIS1 cooperate in peroxisome division independently of mitochondrial fission factor. Journal of Cell Science, 135(13), jcs259924.

Shi, W., Tan, C., Liu, C., & Chen, D. (2022). Mitochondrial fission mediated by Drp1-Fis1 pathway and neurodegenerative diseases. Reviews in the Neurosciences.

Egner, J. M., Nolden, K. A., Harwig, M. C., Bonate, R. P., De Anda, J., Tessmer, M. H., ... & Hill, R. B. (2022). Structural studies of human fission protein FIS1 reveal a dynamic region important for GTPase DRP1 recruitment and mitochondrial fission. Journal of Biological Chemistry, 298(12).

Yu, Y., Peng, X. D., Qian, X. J., Zhang, K. M., Huang, X., Chen, Y. H., ... & Zhu, X. F. (2021). Fis1 phosphorylation by Met promotes mitochondrial fission and hepatocellular carcinoma metastasis. Signal Transduction and Targeted Therapy, 6(1), 401.

Kim, D., Sankaramoorthy, A., & Roy, S. (2020). Downregulation of Drp1 and Fis1 inhibits mitochondrial fission and prevents high glucose-induced apoptosis in retinal endothelial cells. Cells, 9(7), 1662.

Yu, R., Jin, S. B., Lendahl, U., Nistér, M., & Zhao, J. (2019). Human Fis1 regulates mitochondrial dynamics through inhibition of the fusion machinery. The EMBO journal, 38(8), e99748.

Scarpelli, P. H., Tessarin‐Almeida, G., Viçoso, K. L., Lima, W. R., Borges‐Pereira, L., Meissner, K. A., ... & Garcia, C. R. (2019). Melatonin activates FIS 1, DYN 1, and DYN 2 Plasmodium falciparum related‐genes for mitochondria fission: Mitoemerald‐GFP as a tool to visualize mitochondria structure. Journal of pineal research, 66(2), e12484.

Joshi, A. U., Saw, N. L., Shamloo, M., & Mochly-Rosen, D. (2018). Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget, 9(5), 6128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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