Mouse Anti-FMN1 Recombinant Antibody (4F4) (V2LY-0925-LY2396)

Name: Mouse Anti-FMN1 Recombinant Antibody (4F4)
SKU: V2LY-0925-LY2396
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4F4

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-109 representing partial length of human Formin 1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

formin 1

Entrez Gene ID

342184

UniProt ID

Q68DA7

Function

Plays a role in the formation of adherens junction and the polymerization of linear actin cables.

Biological Process

Actin nucleation Source: InterPro
Forelimb morphogenesis Source: Ensembl
Gene expression Source: Ensembl
Hindlimb morphogenesis Source: Ensembl
Positive regulation of actin nucleation Source: Ensembl
Positive regulation of focal adhesion assembly Source: Ensembl
Skeletal system morphogenesis Source: Ensembl
Ureteric bud invasion Source: Ensembl

Cellular Location

Cell membrane; Cytoplasm; Nucleus; Adherens junction. Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions and to microtubules (By similarity).

PTM

Phosphorylated on serine and possibly threonine residues.

MAITI, S., & Das, S. (2023). Probing the ligand binding specificity of FNBP4 WW domains and interaction with FH1 domain of FMN1. bioRxiv, 2023-02.

Das, S. (2023). Probing the ligand binding specificity of FNBP4 WW domains and interaction with FH1 domain of FMN1.

Romero, M. D., & Carabeo, R. A. (2022). Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry. Journal of cell science, 135(19), jcs260185.

Monzo, P., Crestani, M., Chong, Y. K., Ghisleni, A., Hennig, K., Li, Q., ... & Gauthier, N. C. (2021). Adaptive mechanoproperties mediated by the formin FMN1 characterize glioblastoma fitness for invasion. Developmental cell, 56(20), 2841-2855.

Romero, M. D., & Carabeo, R. A. (2021). The Chlamydia trachomatis type III effector TarP coordinates a functional collaboration between the actin nucleators Formin 1 and Arp2/3 during invasion. bioRxiv, 2021-03.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-FMN1 Recombinant Antibody (CBXF-1757) (CAT#: V2LY-0925-LY2397)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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