Mouse Anti-FOLH1 Recombinant Antibody (CBXF-0805) (V2LY-0925-LY2557)

Name: Mouse Anti-FOLH1 Recombinant Antibody (CBXF-0805)
SKU: V2LY-0925-LY2557
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FOLH1 Recombinant Antibody (CBXF-0805) (V2LY-0925-LY2557) in IHC

Primary tumor of one patient with (A) EGFR and (B) VEGF expression but absence of (C) prostate-specific membrane antigen (PSMA) and (D) EpCAM expression. Original magnification 200×. ...View More

van der Fels, C. A., Palthe, S., Buikema, H., van den Heuvel, M. C., Leliveld, A., & de Jong, I. J. (2020). Potential receptors for targeted imaging of lymph node metastases in penile cancer. Diagnostics, 10(9), 694.

Mouse Anti-FOLH1 Recombinant Antibody (CBXF-0805) (V2LY-0925-LY2557) in IHC

Lymph node metastasis of one patient with expression of (A) EGFR; (B) VEGF; no expression of (C) PSMA and (D) EpCAM. Original magnification 200×. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0805

Application

WB, IP, IF, IHC-P

Immunogen

Crude membrane protein preparation from pooled human prostate malignant carcinoma.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Glutamate carboxypeptidase 2

Entrez Gene ID

2346

UniProt ID

Q04609

Function

Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.

Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Biological Process

C-terminal protein deglutamylation Source: BHF-UCL
Proteolysis Source: BHF-UCL

Cellular Location

Cell membrane
Isoform PSMA': Cytoplasm

Topology

Cytoplasmic: 1-19
Helical: 20-43
Extracellular: 44-750

PTM

The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.

Sun, D., Zhang, A., Gao, B., Zou, L., Huang, H., Zhao, X., & Xu, D. (2022). Identification of Alternative Splicing-Related Genes CYB561 and FOLH1 in the Tumor-Immune Microenvironment for Endometrial Cancer Based on TCGA Data Analysis. Frontiers in Genetics, 13.

Ramirez‐Fort, M. K., Meier‐Schiesser, B., Lachance, K., Mahase, S. S., Church, C. D., Niaz, M. J., ... & Bander, N. H. (2021). Folate hydrolase‐1 (FOLH1) is a novel target for antibody‐based brachytherapy in Merkel cell carcinoma. Skin health and disease, 1(1), e9.

Zink, C. F., Barker, P. B., Sawa, A., Weinberger, D. R., Wang, M., Quillian, H., ... & Bigos, K. L. (2020). Association of missense mutation in FOLH1 with decreased NAAG levels and impaired working memory circuitry and cognition. American Journal of Psychiatry, 177(12), 1129-1139.

Bahler, C. D., Johnson, M. M., Davicioni, E., Zhang, J., Cheng, L., Green, M. A., & Koch, M. O. (2020). Predictors of Prostate-specific Membrane Antigen (PSMA/FOLH1) Expression in a Genomic Database. Urology, 144, 117-122.

Kwan, E. M., To, S. Q., Fettke, H. C., Docanto, M. M., Bukczynska, P., Mant, A. M., ... & Azad, A. (2019). Whole blood FOLH1 mRNA expression and treatment response in metastatic castration-resistant prostate cancer (mCRPC).

Šilhavý, J., Marková, I., Hüttl, M., Malínská, H., Kazdová, L., Liška, F., ... & Oliyarnyk, O. (2018). Dissecting the role of Folr1 and Folh1 genes in the pathogenesis of metabolic syndrome in spontaneously hypertensive rats. Physiological research, 67(4), 657-662.

Paul, S., Sadhukhan, S., Munian, D., Bankura, B., & Das, M. (2018). Association of FOLH1, DHFR, and MTHFR gene polymorphisms with susceptibility of Neural Tube Defects: A case control study from Eastern India. Birth Defects Research, 110(14), 1129-1138.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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