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Mouse Anti-FOXA2 Recombinant Antibody (CBXF-0829) (CBMAB-F3197-CQ)

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Published Data
CBMAB-F3197-CQ-1
Mouse Anti-FOXA2 Recombinant Antibody (CBXF-0829) (CBMAB-F3197-CQ) in IF
Localization patterns of germ layer markers: endoderm (FOXA2), mesoendoderm (T), and primitive ectoderm/neuroepithelium (SOX2) in 56-h old ME-primed 𝜇NETs generated from control iPSCs and diseased iPSCs. Images are cross-sectional views of 3D confocal sections (top panel) and magnified cross-sectional side views of 𝜇NETs (bottom panel) illustrate a loss of 3D folding in the diseased 𝜇NETs as compared to control 𝜇NETs while retaining trilaminar germ layer organization. ...View More
Sahni, G., Chang, S. Y., Meng, J. T. C., Tan, J. Z. Y., Fatien, J. J. C., Bonnard, C., ... & Toh, Y. C. (2021). A micropatterned human-specific Neuroepithelial tissue for modeling gene and Drug-induced neurodevelopmental defects. Advanced Science, 8(5), 2001100.
CBMAB-F3197-CQ-2
Mouse Anti-FOXA2 Recombinant Antibody (CBXF-0829) (CBMAB-F3197-CQ) in IF
A-
C) Localization patterns of germ layer markers: endoderm (FOXA2), mesoendoderm (T), and neuroepithelium (SOX2) in ME-primed 𝜇NETs generated from normal H1 human embryonic stem cell, an isogenic FMR1 knock-out H1 cell line (FMR1-KO H1) and a FXS human embryonic stem cell line (FXShESC). A) Maximum intensity projections of 3D confocal sections of 56-h old (top panel) and 72-h old (bottom panel) 𝜇NETs generated from normal and diseased cell lines. B) Magnified cross-sectional views of 3D confocal sections. C) Localization map of SOX2+, T+ and FOXA2+ cells along x-z crosssectional plane indicated by white dotted lines in (A), illustrate a loss of laminar organization of SOX2+, T+, and FOXA2+ cells in the FMR1-KO-H1 and FXS-hESC 𝜇NETs as compared to normal H1 𝜇NETs. Data are overlay map of>3 optical sections from two independent experiments. ...View More
Sahni, G., Chang, S. Y., Meng, J. T. C., Tan, J. Z. Y., Fatien, J. J. C., Bonnard, C., ... & Toh, Y. C. (2021). A micropatterned human-specific Neuroepithelial tissue for modeling gene and Drug-induced neurodevelopmental defects. Advanced Science, 8(5), 2001100.
CBMAB-F3197-CQ-3
Mouse Anti-FOXA2 Recombinant Antibody (CBXF-0829) (CBMAB-F3197-CQ) in WB
Expression of pluripotency markers (OCT4 and NANOG) and endoderm differentiation markers (SOX17 and FOXA2) in the definitive endoderm (DE) of HCOs and untreated hESCs as detected via qRT-PCR. SOX17 and FOXA2 expression was detected by Western blotting, with GAPDH used as a loading control. ...View More
Park, C. S., Nguyen, L. P., & Yong, D. (2020). Development of colonic organoids containing enteric nerves or blood vessels from human embryonic stem cells. Cells, 9(10), 2209.
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBXF-0829
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 431-459 at the C-terminus of mouse HNF-3β.
Specificity
Human, Mouse, Rat, Horse, Dog, Cattle, Pig
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Forkhead Box A2
Introduction
This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.
Entrez Gene ID
Human3170
Mouse15376
Rat25099
UniProt ID
HumanQ9Y261
MouseP35583
RatP32182
Alternative Names
Forkhead Box A2; Forkhead Box Protein A2; Transcription Factor 3B; HNF-3-Beta; HNF-3B; TCF-3B;
Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity).

In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Biological Process
Adult locomotory behavior Source: ParkinsonsUK-UCL
Anatomical structure morphogenesis Source: GO_Central
Cell differentiation Source: GO_Central
Cell fate specification Source: ParkinsonsUK-UCL
Chromatin organization Source: UniProtKB-KW
Dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
Endocrine pancreas development Source: BHF-UCL
Negative regulation of detection of glucose Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of epithelial to mesenchymal transition Source: BHF-UCL
Negative regulation of glucokinase activity Source: BHF-UCL
Negative regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
Positive regulation of cell-cell adhesion mediated by cadherin Source: BHF-UCL
Positive regulation of embryonic development Source: UniProtKB
Positive regulation of gastrulation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription from RNA polymerase II promoter by glucose Source: BHF-UCL
Primitive streak formation Source: UniProtKB
Regulation of blood coagulation Source: UniProtKB
Regulation of insulin secretion involved in cellular response to glucose stimulus Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: BHF-UCL
Response to interleukin-6 Source: UniProtKB
Cellular Location
Cytoplasm; Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
PTM
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.

Gao, X. Q., Liu, C. Y., Zhang, Y. H., Wang, Y. H., Zhou, L. Y., Li, X. M., ... & Wang, K. (2022). The circRNA CNEACR regulates necroptosis of cardiomyocytes through Foxa2 suppression. Cell Death & Differentiation, 29(3), 527-539.

Kumar, A., Sundaram, K., Teng, Y., Mu, J., Sriwastva, M. K., Zhang, L., ... & Zhang, H. G. (2022). Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance. Theranostics, 12(3), 1388.

Han, M., Li, F., Zhang, Y., Dai, P., He, J., Li, Y., ... & Gao, D. (2022). FOXA2 drives lineage plasticity and KIT pathway activation in neuroendocrine prostate cancer. Cancer Cell, 40(11), 1306-1323.

Sahoo, S. S., Ramanand, S. G., Gao, Y., Abbas, A., Kumar, A., Cuevas, I. C., ... & Castrillon, D. H. (2022). FOXA2 suppresses endometrial carcinogenesis and epithelial-mesenchymal transition by regulating enhancer activity. The Journal of Clinical Investigation, 132(12).

Liu, X., Hou, S., Xiang, R., Hu, C., Chen, Z., Li, N., ... & Yang, J. (2022). Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis. Metabolism, 136, 155292.

Liu, Q., Pang, J., Wang, L. A., Huang, Z., Xu, J., Yang, X., ... & Jiang, J. (2021). Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2. The Journal of Pathology, 253(1), 106-118.

Choi, W., Choe, S., & Lau, G. W. (2020). Inactivation of FOXA2 by respiratory bacterial pathogens and dysregulation of pulmonary mucus homeostasis. Frontiers in immunology, 11, 515.

Lee, K., Cho, H., Rickert, R. W., Li, Q. V., Pulecio, J., Leslie, C. S., & Huangfu, D. (2019). FOXA2 is required for enhancer priming during pancreatic differentiation. Cell reports, 28(2), 382-393.

Cernilogar, F. M., Hasenöder, S., Wang, Z., Scheibner, K., Burtscher, I., Sterr, M., ... & Schotta, G. (2019). Pre-marked chromatin and transcription factor co-binding shape the pioneering activity of Foxa2. Nucleic Acids Research, 47(17), 9069-9086.

Zhu, L., An, L., Ran, D., Lizarraga, R., Bondy, C., Zhou, X., ... & Chen, Y. (2019). The club cell marker SCGB1A1 downstream of FOXA2 is reduced in asthma. American journal of respiratory cell and molecular biology, 60(6), 695-704.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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