FOXA2 Antibodies

Structure of FOXA2

FOXA2 is a relatively small transcription factor with a molecular weight of approximately 47.8 kDa. This value varies slightly among different species, mainly due to minor changes in the amino acid sequence.

This protein contains approximately 457 amino acids and its core feature is a highly conserved forkhead domain. This domain consists of three α-helices and two large ring-like structures, forming a typical helix-ring-helix-folding pattern. Structural biology analysis shows that this domain achieves sequence-specific binding by recognizing and inserting into the major groove of the DNA double helix. The two ring-like regions (wings) in the winged helix structure interact stably with the DNA backbone, while the third α-helix directly embeds into the recognition site to ensure the precise positioning of the target gene promoter.

Fig. 1 Schematic diagram of FOXA2.¹

Key structural properties of FOXA2:

• The fork-head domain consists of three α -helical rings and two wing-shaped rings
• Identify the helix directly embedded in the major groove of DNA
• The C-terminal region is rich in basic amino acids and is involved in nuclear localization
• N-terminal region mediated chromatin remodeling, auxiliary open target genes

Functions of FOXA2

The main function of FOXA2 is to regulate the development of the endoderm and metabolic homeostasis. Additionally, it is involved in several physiological processes, including glucose homeostasis maintenance, cell proliferation, and organ formation.

The binding kinetics of FOXA2 to DNA exhibits a synergistic effect. Its affinity for the nucleosome binding site is significantly higher than that of other transcription factors, reflecting its unique biological status as a chromatin opening factor.

Applications of FOXA2 and FOXA2 Antibody in Literature

1. Choi, Woosuk, Shawn Choe, and Gee W. Lau. "Inactivation of FOXA2 by respiratory bacterial pathogens and dysregulation of pulmonary mucus homeostasis." Frontiers in immunology 11 (2020): 515. https://doi.org/10.3389/fimmu.2020.00515

The article indicates that FOXA2 is a key factor regulating the mucous homeostasis of the airway. In a healthy state, it inhibits the differentiation of goblet cells and the production of mucin; during infection, its expression is downregulated, leading to excessive mucus secretion, clearance disorders, and persistent infection, thereby accelerating the progression of chronic lung diseases.

2. Belaguli, Narasimhaswamy S., et al. "Forkhead box protein A2 (FOXA2) protein stability and activity are regulated by sumoylation." PloS one 7.10 (2012): e48019. https://doi.org/10.1371/journal.pone.0048019

The study found that the FOXA2 protein is modified by SUMO-1 at the site K6. This modification promotes the stability and transcriptional activity of the FOXA2 protein, and is crucial for maintaining its expression and function in various cancer cells.

3. Kawasaki, Kenta, et al. "FOXA2 promotes metastatic competence in small cell lung cancer." Nature Communications 16.1 (2025): 4865. https://doi.org/10.1038/s41467-025-60141-5

The research has found that FOXA2 is a key driver for multi-organ metastasis in small cell lung cancer (SCLC). ASCL1 directly regulates the expression of FOXA2 by binding to its promoter, thereby inducing the fetal neuroendocrine gene program and significantly promoting distant metastasis of the tumor.

4. Wang, Zifeng, et al. "FOXA2 rewires AP-1 for transcriptional reprogramming and lineage plasticity in prostate cancer." Nature Communications 15.1 (2024): 4914. https://doi.org/10.1038/s41467-024-49234-9

Studies have shown that FOXA2 is a core transcription factor that regulates the development and diseases of multiple organs. In the lungs, it maintains mucus homeostasis, and its absence leads to goblet cell hyperplasia and infection. In cancer cells, FOXA2 is stabilized through SUMO modification and driven by ASCL1, collaborating with AP-1 to reprogram genes and jointly promoting the metastasis of small cell lung cancer and the plasticity of prostate cancer lineage.

5. Kumar, Anil, et al. "Ginger nanoparticles mediated induction of Foxa2 prevents high-fat diet-induced insulin resistance." Theranostics 12.3 (2022): 1388. https://doi.org/10.7150/thno.62514

Studies have shown that oral administration of ginger-derived nanoparticles (GDNP) effectively prevents obesity and insulin resistance induced by a high-fat diet and extends the lifespan of mice by protecting the intestinal epithelial FOXA2 from Akt-1-mediated phosphorylation inactivation, thereby restoring its function and maintaining signal homeostasis.

Creative Biolabs: FOXA2 Antibodies for Research

Creative Biolabs specializes in the production of high-quality FOXA2 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom FOXA2 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our FOXA2 antibodies, custom preparations, or technical support, contact us at email.