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Rabbit Anti-FPGS Recombinant Antibody (CBXF-1086) (CBMAB-F0969-CQ)

This product is a rabbit antibody that recognizes FPGS. The antibody CBXF-1086 can be used for immunoassay techniques such as: WB, FC.
See all FPGS antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBXF-1086
Antibody Isotype
IgG
Application
WB, FC

Basic Information

Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Folylpolyglutamate Synthase
Introduction
This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms.
Entrez Gene ID
Human2356
Mouse14287
Rat687266
UniProt ID
HumanQ05932
MouseP48760
RatM0R401
Alternative Names
Folylpolyglutamate Synthase; EC 6.3.2.17; Tetrahydrofolylpolyglutamate Synthase; Folylpoly-Gamma-Glutamate Synthetase; Tetrahydrofolate Synthase;
Function
Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.
Biological Process
Animal organ regeneration Source: Ensembl
Brain development Source: Ensembl
Cell population proliferation Source: BHF-UCL
Folic acid-containing compound biosynthetic process Source: GO_Central
Folic acid-containing compound metabolic process Source: BHF-UCL
Folic acid metabolic process Source: Reactome
Glutamate metabolic process Source: BHF-UCL
Liver development Source: Ensembl
Nucleobase-containing compound metabolic process Source: ProtInc
One-carbon metabolic process Source: UniProtKB-KW
Tetrahydrofolylpolyglutamate biosynthetic process Source: BHF-UCL
Cellular Location
Isoform 1: Mitochondrion inner membrane; Mitochondrion matrix
Isoform 2: Cytoplasm

Stark, M., Levin, M., Ulitsky, I., & Assaraf, Y. G. (2023). Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion. BMC biology, 21(1), 1-23.

Muller, I. B., Lin, M., Lems, W. F., Ter Wee, M. M., Wojtuszkiewicz, A., Nurmohamed, M. T., ... & de Jonge, R. (2021). Association of altered folylpolyglutamate synthetase pre-mRNA splicing with methotrexate unresponsiveness in early rheumatoid arthritis. Rheumatology, 60(3), 1273-1281.

Yu, S. L., Zhang, H., Ho, B. C., Yu, C. H., Chang, C. C., Hsu, Y. C., ... & Yang, Y. L. (2020). FPGS relapse-specific mutations in relapsed childhood acute lymphoblastic leukemia. Scientific Reports, 10(1), 12074.

Schmidt, T. T., Sharma, S., Reyes, G. X., Kolodziejczak, A., Wagner, T., Luke, B., ... & Hombauer, H. (2020). Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio. Nucleic acids research, 48(1), 264-277.

Maezawa, Y., Sakamaki, K., Oue, N., Kimura, Y., Hashimoto, I., Hara, K., ... & Oshima, T. (2020). High gamma-glutamyl hydrolase and low folylpolyglutamate synthetase expression as prognostic biomarkers in patients with locally advanced gastric cancer who were administrated postoperative adjuvant chemotherapy with S-1. Journal of Cancer Research and Clinical Oncology, 146, 75-86.

Mazarei, M., Baxter, H. L., Srivastava, A., Li, G., Xie, H., Dumitrache, A., ... & Stewart Jr, C. N. (2020). Silencing folylpolyglutamate synthetase1 (FPGS1) in switchgrass (Panicum virgatum L.) improves lignocellulosic biofuel production. Frontiers in Plant Science, 11, 843.

Muller, I. B., Lin, M., Struys, E. A., Heydari, P., Hebing, R. C., Nurmohamed, M. T., ... & de Jonge, R. (2019). Development and validation of a sensitive UHPLC-MS/MS–based method for the analysis of folylpolyglutamate synthetase enzymatic activity in peripheral blood mononuclear cells: application in rheumatoid arthritis and leukemia patients. Therapeutic Drug Monitoring, 41(5), 598-606.

Kodidela, S., Pradhan, S. C., Dubashi, B., & Basu, D. (2018). Interethnic differences in single and haplotype structures of folylpolyglutamate synthase and gamma-glutamyl hydrolase variants and their influence on disease susceptibility to acute lymphoblastic leukemia in the Indian population: An exploratory study. Indian Journal of Medical and Paediatric Oncology, 39(03), 331-338.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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