Rabbit Anti-FSCN1 Recombinant Antibody (D1A8) (CBMAB-CP0682-LY)

Name: Rabbit Anti-FSCN1 Recombinant Antibody (D1A8)
SKU: CBMAB-CP0682-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D1A8

Application

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg201 of human fascin protein.

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fascin Actin-Bundling Protein 1

Introduction

This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]

Entrez Gene ID

Human	6624
Mouse	14086
Monkey	100430727

UniProt ID

Human	Q16658
Mouse	Q61553
Monkey	F7EX32

Alternative Names

Fascin Actin-Bundling Protein 1; 55 KDa Actin-Bundling Protein; FAN1; P55; SNL; HSN; Fascin Homolog 1; Actin-Bundling Protein (Strongylocentrotus Purpuratus); Singed (Drosophila)-Like (Sea Urchin Fascin Homolog Like);

Function

Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945).

Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945).

Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786).

Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945).

Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786).

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Actin filament bundle assembly Source: UniProtKB
Cell-cell junction assembly Source: UniProtKB
Cell migration Source: UniProtKB
Cell motility Source: UniProtKB
Establishment of apical/basal cell polarity Source: UniProtKB
Establishment or maintenance of cell polarity Source: GO_Central
Microspike assembly Source: UniProtKB
Parallel actin filament bundle assembly Source: UniProtKB
Positive regulation of extracellular matrix disassembly Source: UniProtKB
Positive regulation of filopodium assembly Source: UniProtKB
Positive regulation of lamellipodium assembly Source: UniProtKB
Positive regulation of podosome assembly Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of microvillus assembly Source: UniProtKB

Cellular Location

Cytoskeleton; Stress fiber; Cytosol; Cell cortex; Filopodium; Invadopodium; Microvillus; Cell junction. Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity).

PTM

Phosphorylation at Ser-39 inhibits actin-binding (PubMed:8647875, PubMed:8999969). Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF (PubMed:22155786).

Li, Z., Shi, J., Zhang, N., Zheng, X., Jin, Y., Wen, S., ... & Gao, W. (2022). FSCN1 acts as a promising therapeutic target in the blockade of tumor cell motility: a review of its function, mechanism, and clinical significance. Journal of Cancer, 13(8), 2528.

Shi, Y., Xu, Y., Xu, Z., Wang, H., Zhang, J., Wu, Y., ... & Wang, K. (2022). TKI resistant-based prognostic immune related gene signature in LUAD, in which FSCN1 contributes to tumor progression. Cancer Letters, 532, 215583.

Tang, J., Xu, H., Liu, Q., Zheng, J., Pan, C., Li, Z., ... & Chen, L. (2021). LncRNA LOC146880 promotes esophageal squamous cell carcinoma progression via miR-328-5p/FSCN1/MAPK axis. Aging (Albany NY), 13(10), 14198.

Xu, M. L., Liu, T. C., Dong, F. X., Meng, L. X., Ling, A. X., & Liu, S. (2021). Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis. Aging (Albany NY), 13(15), 19776.

Wu, Y., Yuan, M. H., Wu, H. T., Chen, W. J., Zhang, M. L., Ye, Q. Q., ... & Zhang, G. J. (2020). MicroRNA-488 inhibits proliferation and motility of tumor cells via downregulating FSCN1, modulated by Notch3 in breast carcinomas. Cell Death & Disease, 11(10), 912.

Ou, C., Sun, Z., He, X., Li, X., Fan, S., Zheng, X., ... & Ma, J. (2020). Targeting YAP1/LINC00152/FSCN1 signaling axis prevents the progression of colorectal cancer. Advanced science, 7(3), 1901380.

Li, X., Han, X., Wei, P., Yang, J., & Sun, J. (2020). Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1. Cancer Biology & Therapy, 21(5), 452-462.

Gao, W., Zhang, C., Li, W., Li, H., Sang, J., Zhao, Q., ... & Wang, B. (2019). Promoter methylation-regulated miR-145-5p inhibits laryngeal squamous cell carcinoma progression by targeting FSCN1. Molecular therapy, 27(2), 365-379.

Shen, S. N., Li, K., Liu, Y., Yang, C. L., He, C. Y., & Wang, H. R. (2019). Retracted: Down‐regulation of long noncoding RNA PVT 1 inhibits esophageal carcinoma cell migration and invasion and promotes cell apoptosis via micro RNA‐145‐mediated inhibition of FSCN 1. Molecular oncology, 13(12), 2554-2573.

Gao, R., Zhang, N., Yang, J., Zhu, Y., Zhang, Z., Wang, J., ... & Bi, J. (2019). Long non-coding RNA ZEB1-AS1 regulates miR-200b/FSCN1 signaling and enhances migration and invasion induced by TGF-β1 in bladder cancer cells. Journal of Experimental & Clinical Cancer Research, 38(1), 1-15.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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