Mouse Anti-FTH1 Recombinant Antibody (
18C10) (V2LY-0725-LY714)

Name: Mouse Anti-FTH1 Recombinant Antibody (18C10)
SKU: V2LY-0725-LY714
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-FTH1 Recombinant Antibody (18C10) (V2LY-0725-LY714) in WB

Western Blot
Positive WB detected in: U251 whole cell lysate,JK whole cell lysate
All lanes: FTH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

Mouse Anti-FTH1 Recombinant Antibody (18C10) (V2LY-0725-LY714) in IHC

IHC image of V2LY-0725-LY714 diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-FTH1 Recombinant Antibody (18C10) (V2LY-0725-LY714) in IHC

IHC image of V2LY-0725-LY714 diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Mouse Anti-FTH1 Recombinant Antibody (18C10) (V2LY-0725-LY714) in FC

Overlay Peak curve showing 293 cells stained with V2LY-0725-LY714 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

18C10

Application

ELISA, WB, IHC, FC

Immunogen

Recombinant Human FTH1 protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000-1:5,000
IHC	1:20-1:200
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ferritin Heavy Chain 1

Entrez Gene ID

2495

UniProt ID

P02794

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Biological Process

Cellular iron ion homeostasis Source: ProtInc
Immune response Source: UniProtKB
Intracellular sequestering of iron ion Source: UniProtKB
Iron ion transport Source: GO_Central
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of fibroblast proliferation Source: UniProtKB

Cellular Location

Cytosol; Extracellular exosome; Extracellular region; Autolysosome; Nucleus; Cytoplasm; Ficolin-1-rich granule lumen; Intracellular ferritin complex; Tertiary granule lumen

Involvement in disease

The disease is caused by variants affecting the gene represented in this entry. In a Japanese family affected by HFE5, a single point mutation has been detected in the iron-responsive element (IRE) in the 5'-UTR of FTH1 mRNA. This mutation leads to an increased binding affinity for iron regulatory protein and thereby to the efficient suppression of mRNA translation. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.

Yang, G., Pan, Q., Lu, Y., Zhu, J., & Gou, X. (2023). miR-29a-5p modulates ferroptosis by targeting ferritin heavy chain FTH1 in prostate cancer. Biochemical and Biophysical Research Communications.

Muhammad, J. S., ElGhazali, G., Shafarin, J., Mohammad, M. G., Abu-Qiyas, A., & Hamad, M. (2022). SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients. Biochemical and Biophysical Research Communications, 631, 138-145.

Ali, A., Shafarin, J., Abu Jabal, R., Aljabi, N., Hamad, M., Sualeh Muhammad, J., ... & Hamad, M. (2021). Ferritin heavy chain (FTH1) exerts significant antigrowth effects in breast cancer cells by inhibiting the expression of c‐MYC. FEBS Open bio, 11(11), 3101-3114.

Li, X., Si, W., Li, Z., Tian, Y., Liu, X., Ye, S., ... & Zhu, M. (2021). miR‑335 promotes ferroptosis by targeting ferritin heavy chain 1 in in vivo and in vitro models of Parkinson's disease. International Journal of Molecular Medicine, 47(4), 1-12.

Bertoli, S., Paubelle, E., Bérard, E., Saland, E., Thomas, X., Tavitian, S., ... & Récher, C. (2019). Ferritin heavy/light chain (FTH1/FTL) expression, serum ferritin levels, and their functional as well as prognostic roles in acute myeloid leukemia. European journal of haematology, 102(2), 131-142.

Ravi, V., Madhankumar, A. B., Abraham, T., Slagle-Webb, B., & Connor, J. R. (2019). Liposomal delivery of ferritin heavy chain 1 (FTH1) siRNA in patient xenograft derived glioblastoma initiating cells suggests different sensitivities to radiation and distinct survival mechanisms. PLoS One, 14(9), e0221952.

Huang, H., Qiu, Y., Huang, G., Zhou, X., Zhou, X., & Luo, W. (2019). Value of ferritin heavy chain (FTH1) expression in diagnosis and prognosis of renal cell carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 25, 3700.

Balaratnam, S., West, N., & Basu, S. (2018). A piRNA utilizes HILI and HIWI2 mediated pathway to down-regulate ferritin heavy chain 1 mRNA in human somatic cells. Nucleic Acids Research, 46(20), 10635-10648.

Jin, P., Kang, J., Lee, M. K., & Park, J. W. (2018). Ferritin heavy chain controls the HIF-driven hypoxic response by activating the asparaginyl hydroxylase FIH. Biochemical and biophysical research communications, 499(3), 475-481.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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