Mouse Anti-FUT4 Monoclonal Antibody (1) (CBMAB-F2723-CQ)

Name: Mouse Anti-FUT4 Monoclonal Antibody (1)
SKU: CBMAB-F2723-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FUT4 Recombinant Antibody (1) (CBMAB-F2723-CQ) in IHC

Immunolocalization of FUCA1 and Lewis-x antigens in different stages of human breast cancer. The tumor tissues were cut into 5-µm serial sections and stained with antibodies specific to human FUCA1 or fucosylated Lewis-x antigen. H.E., hematoxylin and eosin stain. Scale bar = 200 µm. ...View More

Cheng, T. C., Tu, S. H., Chen, L. C., Chen, M. Y., Chen, W. Y., Lin, Y. K., Ho, C. T., Lin, S. Y., Wu, C. H., & Ho, Y. S. (2015). Down-regulation of α-L-fucosidase 1 expression confers inferior survival for triple-negative breast cancer patients by modulating the glycosylation status of the tumor cell surface. Oncotarget, 6(25), 21283-21300.

Mouse Anti-FUT4 Recombinant Antibody (1) (CBMAB-F2723-CQ) in IF

At 3 DIV, stage-specific embryonic antigen 1 (SSEA-1) (green), a cell surface carbohydrate epitope found on uncommitted NSPCs, appeared to localize to the plasma membrane of cells with undifferentiated morphology. All scale bars: 20 μm. ...View More

Chan, W. S., Sideris, A., Sutachan, J. J., Montoya G, J. V., Blanck, T. J., & Recio-Pinto, E. (2013). Differential regulation of proliferation and neuronal differentiation in adult rat spinal cord neural stem/progenitors by ERK1/2, Akt, and PLCγ. Frontiers in molecular neuroscience, 6, 23.

Mouse Anti-FUT4 Recombinant Antibody (1) (CBMAB-F2723-CQ) in IF

Induction and characterization of PVi lines.

(A) Protocol for reprogramming PVEFs. All brightfield and immunolabeling images (B, C, E-J) depict colonies of a single representative PVi line (line 6). (B-C) Colony morphology of a PVi line. Colonies display morphology similar to that of mouse ES cells, including distinct raised colonies (B), with tightly-packed cells and well-defined, phase-bright margins (C). (D) RT-PCR for endogenous reprogramming factors in PVi lines. RT-PCR for pv-Nat1 was performed as a positive control for an endogenous, ubiquitously expressed gene that should be expressed in all cells irrespective of their reprogramming state. Lanes 1-11 show PCR products from PVi lines 1-11, respectively. Lane 12 ("F") shows PCR products from PVEFs. (E) PVi colony exhibiting alkaline phosphatase (Alk. phosphatase) activity. (F) Live immunofluorescent labeling of a PVi colony for SSEA-1. (G-J) Immunofluorescent labeling of PVi colonies for Nanog, Oct3/4, Klf4, and Sox2. Scale bars equal 500 µm (B), 100 µm (C, E), and 50 µm (F-J). ...View More

Manoli, D. S., Subramanyam, D., Carey, C., Sudin, E., Van Westerhuyzen, J. A., Bales, K. L., Blelloch, R., & Shah, N. M. (2012). Generation of induced pluripotent stem cells from the prairie vole. PloS one, 7(5), e38119.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

FC, IF, IHC, IP, WB

Immunogen

Testis embryonal carcinoma F9 cell line.

Specificity

Human, Mouse, Rat, Cattle, Chicken, Fruit fly, Horse

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.2-0.5 µg/ml
IF(ICC)	2-5 µg/ml
IHC	2-5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fucosyltransferase 4

Introduction

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).

Entrez Gene ID

2526

UniProt ID

P22083

Alternative Names

Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; Alpha (1,3) Fucosyltransferase, Myeloid-Specific; ELAM-1 Ligand Fucosyltransferase; ELAM Ligand Fucosyltransferase; Fucosyltransferase IV; FUC-TIV; FucT-IV; FCT3A; ELFT; Stage-Specific Embryonic Antigen 1;

Function

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

Biological Process

Carbohydrate metabolic process Source: ProtInc
Fucosylation Source: GO_Central
L-fucose catabolic process Source: UniProtKB
Oligosaccharide biosynthetic process Source: Reactome
Oligosaccharide metabolic process Source: UniProtKB
Positive regulation of leukocyte tethering or rolling Source: UniProtKB
Protein glycosylation Source: UniProtKB
Regulation of leukocyte cell-cell adhesion Source: UniProtKB

Cellular Location

Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.

Topology

Cytoplasmic: 1-147
Helical: 148-172
Lumenal: 173-530

Aziz, F., Khan, I., Shukla, S., Dey, D. K., Yan, Q., Chakraborty, A., ... & Han, Y. K. (2022). Partners in crime: The Lewis Y antigen and fucosyltransferase IV in Helicobacter pylori-induced gastric cancer. Pharmacology & therapeutics, 232, 107994.

Lu, H. H., Lin, S. Y., Weng, R. R., Juan, Y. H., Chen, Y. W., Hou, H. H., ... & Tsai, H. C. (2020). Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma. EBioMedicine, 57, 102846.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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