Mouse Anti-FUT5 Recombinant Antibody (CBXF-0962) (CBMAB-F3221-CQ)

Name: Mouse Anti-FUT5 Recombinant Antibody (CBXF-0962)
SKU: CBMAB-F3221-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0962

Application

S-ELISA, ELISA, WB

Immunogen

FUT5 (NP_002025, 95 a.a.-164 a.a) partial recombinant protein with GST tag

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

fucosyltransferase 5 (alpha (1,3) fucosyltransferase)

Introduction

FUT5 (Fucosyltransferase 5) is a Protein Coding gene. Among its related pathways are Metabolism and Glycosphingolipid biosynthesis - lacto and neolacto series. Gene Ontology (GO) annotations related to this gene include fucosyltransferase activity and 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity. An important paralog of this gene is FUT3.

Entrez Gene ID

2527

UniProt ID

Q11128

Alternative Names

Fucosyltransferase 5; Galactoside 3-L-Fucosyltransferase; Alpha (1,3) Fucosyltransferase; Fucosyltransferase V; EC 2.4.1.65; FUC-TV; FucT-V; Alpha-(1,3)-Fucosyltransferase 5; EC 2.4.1;

Function

Catalyzes preferentially the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the N-acetyl-beta-D-glucosamine (GlcNAc) of an N-acetyllactosamine unit (type 2 chain) of an oligosacccharide, or a glycoprotein- and a glycolipid-linked N-acetyllactosamine unit via an alpha (1,3) linkage and participates in the surface espression of VIM-2, Lewis X/SSEA-1 and sialyl Lewis X antigens (PubMed:14718375, PubMed:1740457, PubMed:7721776, PubMed:9737988, PubMed:17604274, PubMed:9737989).

Preferentially transfers fucose to the GlcNAc of an internal N-acetyllactosamine unit of a poly-N-acetyllactosamine chain acceptor substrate (PubMed:7721776, PubMed:17604274).

Also catalyzes to a lesser extend the transfer of L-fucose to the GlcNAc of a type 1 (beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl) or H-type 1 (alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-D-GlcNAc) chain oligosacccharide via an alpha (1,4) linkage (PubMed:14718375, PubMed:1740457, PubMed:7721776, PubMed:9737988, PubMed:17604274).

Preferentially catalyzes sialylated type 2 oligosacccharide acceptors over neutral type 2 or H type 2 (alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-D-GlcNAc) oligosacccharide acceptors (PubMed:1740457, PubMed:9737989).

Lactose-based structures are also acceptor substrates (PubMed:1740457, PubMed:7721776).

Biological Process

Carbohydrate metabolic process Source: ProtInc
Ceramide metabolic process Source: UniProtKB
Fucosylation Source: GO_Central
L-fucose catabolic process Source: UniProtKB
Oligosaccharide biosynthetic process Source: Reactome
Oligosaccharide metabolic process Source: UniProtKB
Protein glycosylation Source: UniProtKB

Cellular Location

Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.

Topology

Cytoplasmic: 1-15
Helical: 16-34
Lumenal: 35-374

Fan, R., Han, X., Gong, Y., He, L., Xue, Z., Yang, Y., ... & Zhang, J. (2021). Alterations of fucosyltransferase genes and fucosylated glycans in gastric epithelial cells infected with Helicobacter pylori. Pathogens, 10(2), 168.

Rimkute, I., Thorsteinsson, K., Henricsson, M., Tenge, V. R., Yu, X., Lin, S. C., ... & Larson, G. (2020). Histo-blood group antigens of glycosphingolipids predict susceptibility of human intestinal enteroids to norovirus infection. Journal of Biological Chemistry, 295(47), 15974-15987.

Cid, E., Yamamoto, M., & Yamamoto, F. (2018). Blood group ABO gene–encoded A transferase catalyzes the biosynthesis of FORS1 antigen of FORS system upon Met69Thr/Ser substitution. Blood Advances, 2(12), 1371-1381.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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