Mouse Anti-FXYD1 (Phospho-Ser68) Recombinant Antibody (CBXF-1958) (V2LY-1225-LY340)

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Basic Information

Host Animal
Mouse
Clone
CBXF-1958
Application
WB
Immunogen
Ser68 phosphorylated region of mouse phospholemman.
Host Species
Mouse
Specificity
Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
FXYD Domain Containing Ion Transport Regulator 1
Entrez Gene ID
Mouse56188
Rat58971
UniProt ID
MouseQ9Z239
RatO08589
Function
Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.
Biological Process
Chloride transport Source: ProtInc
Muscle contraction Source: ProtInc
Negative regulation of protein glutathionylation Source: UniProtKB
Positive regulation of sodium ion export across plasma membrane Source: UniProtKB
Potassium ion transport Source: UniProtKB-KW
Regulation of cardiac muscle cell membrane potential Source: BHF-UCL
Regulation of heart contraction Source: BHF-UCL
Regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
Sodium ion transport Source: UniProtKB-KW
Cellular Location
Sarcolemma; Apical cell membrane; T-tubule; Caveola. Detected in the apical cell membrane in brain. In myocytes, localizes to sarcolemma, t-tubules and intercalated disks.
Topology
Extracellular: 21-35
Helical: 36-56
Cytoplasmic: 57-92
PTM
Major plasma membrane substrate for cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation (By similarity). Phosphorylation at Ser-88 stimulates sodium/potassium-transporting ATPase activity while the unphosphorylated form inhibits sodium/potassium-transporting ATPase activity (By similarity). Phosphorylation increases tetramerization, decreases binding to ATP1A1 and reduces inhibition of ATP1A1 activity (By similarity). Phosphorylation at Ser-83 leads to greatly reduced interaction with ATP1A1, ATP1A2 and ATP1A3 (By similarity). May be phosphorylated by DMPK (PubMed:10811636).
Palmitoylation increases half-life and stability and is enhanced upon phosphorylation at Ser-88 by PKA.

Cuomo, M., Florio, E., Della Monica, R., Costabile, D., Buonaiuto, M., Di Risi, T., ... & Chiariotti, L. (2022). Epigenetic remodelling of Fxyd1 promoters in developing heart and brain tissues. Scientific Reports, 12(1), 6471.

Wu, D., Besnier, M., Bubb, K., Di Bartolo, B., Tang, O., & Figtree, G. (2021). FXYD1 Protects Against Pressure-Overload Cardiac Remodelling and Fibrosis. Heart, Lung and Circulation, 30, S124.

Jan, V., Miš, K., Nikolic, N., Dolinar, K., Petrič, M., Bone, A., ... & Pirkmajer, S. (2021). Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+, K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells. Plos one, 16(2), e0247377.

Bubb, K. J., Tang, O., Gentile, C., Moosavi, S. M., Hansen, T., Liu, C. C., ... & Figtree, G. A. (2021). FXYD1 Is Protective Against Vascular Dysfunction. Hypertension, 77(6), 2104-2116.

Yuan, Z. F., Mao, S. S., Shen, J., Jiang, L. H., Xu, L., Xu, J. L., & Gao, F. (2020). Insulin-like growth factor-1 down-regulates the phosphorylation of FXYD1 and rescues behavioral deficits in a mouse model of Rett syndrome. Frontiers in Neuroscience, 14, 20.

Moosavi, S. M., van Reyk, D., Di Bartolo, B., Tang, O., Bubb, K. J., & Figtree, G. A. (2020). Absence of Fxyd1 is Associated With a Female-specific Pro-inflammatory and Hypercholesterolemic Environment: Implications for Atherosclerosis. Circulation, 142(Suppl_3), A16286-A16286.

O’Donnell, A. M., Nakamura, H., Tomuschat, C., Marayati, N. F., & Puri, P. (2019). Abnormal Scn1b and Fxyd1 gene expression in the pulled-through ganglionic colon may influence functional outcome in patients with Hirschsprung’s disease. Pediatric surgery international, 35, 9-14.

Matagne, V., Wondolowski, J., Frerking, M., Shahidullah, M., Delamere, N. A., Sandau, U. S., ... & Ojeda, S. R. (2018). Correcting deregulated Fxyd1 expression rescues deficits in neuronal arborization and potassium homeostasis in MeCP2 deficient male mice. Brain research, 1697, 45-52.

Christiansen, D., Murphy, R. M., Bangsbo, J., Stathis, C. G., & Bishop, D. J. (2018). Increased FXYD1 and PGC‐1α mRNA after blood flow‐restricted running is related to fibre type‐specific AMPK signalling and oxidative stress in human muscle. Acta physiologica, 223(2), e13045.

Bubb, K., Tang, O., Hansen, T., & Figtree, G. (2018). Oxidative modification of the cardiac sodium potassium pump is worsened in the absence of FXYD1, contributing to cardiac dysfunction and fibrosis. Free Radical Biology and Medicine, 128, S21.

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For research use only. Not intended for any clinical use.

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