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Mouse Anti-FXYD1 (Phosphorylated S68) Recombinant Antibody (10i44) (PTM-CBMAB-0259LY)

This antibody is a recombiant antibody against FXYD1. The antibody can be used for immunoassay techniques, such as WB.
See all FXYD1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
10i44
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding Ser68 human PLM
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Supplied as a liquid in PBS, 0.09% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
FXYD Domain Containing Ion Transport Regulator 1
Introduction
This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human5348
Mouse56188
UniProt ID
HumanO00168
MouseQ9Z239
Alternative Names
FXYD Domain Containing Ion Transport Regulator 1; Sodium/Potassium-Transporting ATPase Subunit FXYD1; Phospholemman; PLM; FXYD Domain-Containing Ion Transport Regulator 1;
Function
Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which transports Na+ out of the cell and K+ into the cell. Inhibits NKA activity in its unphosphorylated state and stimulates activity when phosphorylated. Reduces glutathionylation of the NKA beta-1 subunit ATP1B1, thus reversing glutathionylation-mediated inhibition of ATP1B1. Contributes to female sexual development by maintaining the excitability of neurons which secrete gonadotropin-releasing hormone.
Biological Process
Chloride transport Source: ProtInc
Muscle contraction Source: ProtInc
Negative regulation of protein glutathionylation Source: UniProtKB
Positive regulation of sodium ion export across plasma membrane Source: UniProtKB
Potassium ion transport Source: UniProtKB-KW
Regulation of cardiac muscle cell membrane potential Source: BHF-UCL
Regulation of heart contraction Source: BHF-UCL
Regulation of sodium ion transmembrane transporter activity Source: BHF-UCL
Sodium ion transport Source: UniProtKB-KW
Cellular Location
Sarcolemma; Apical cell membrane; T-tubule; Caveola. Detected in the apical cell membrane in brain. In myocytes, localizes to sarcolemma, t-tubules and intercalated disks.
Topology
Extracellular: 21-35
Helical: 36-56
Cytoplasmic: 57-92
PTM
Major plasma membrane substrate for cAMP-dependent protein kinase (PKA) and protein kinase C (PKC) in several different tissues (By similarity). Phosphorylated in response to insulin and adrenergic stimulation (By similarity). Phosphorylation at Ser-88 stimulates sodium/potassium-transporting ATPase activity while the unphosphorylated form inhibits sodium/potassium-transporting ATPase activity (By similarity). Phosphorylation increases tetramerization, decreases binding to ATP1A1 and reduces inhibition of ATP1A1 activity (By similarity). Phosphorylation at Ser-83 leads to greatly reduced interaction with ATP1A1, ATP1A2 and ATP1A3 (By similarity). May be phosphorylated by DMPK (PubMed:10811636).
Palmitoylation increases half-life and stability and is enhanced upon phosphorylation at Ser-88 by PKA.

Cuomo, M., Florio, E., Della Monica, R., Costabile, D., Buonaiuto, M., Di Risi, T., ... & Chiariotti, L. (2022). Epigenetic remodelling of Fxyd1 promoters in developing heart and brain tissues. Scientific Reports, 12(1), 6471.

Wu, D., Besnier, M., Bubb, K., Di Bartolo, B., Tang, O., & Figtree, G. (2021). FXYD1 Protects Against Pressure-Overload Cardiac Remodelling and Fibrosis. Heart, Lung and Circulation, 30, S124.

Jan, V., Miš, K., Nikolic, N., Dolinar, K., Petrič, M., Bone, A., ... & Pirkmajer, S. (2021). Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+, K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells. Plos one, 16(2), e0247377.

Bubb, K. J., Tang, O., Gentile, C., Moosavi, S. M., Hansen, T., Liu, C. C., ... & Figtree, G. A. (2021). FXYD1 Is Protective Against Vascular Dysfunction. Hypertension, 77(6), 2104-2116.

Yuan, Z. F., Mao, S. S., Shen, J., Jiang, L. H., Xu, L., Xu, J. L., & Gao, F. (2020). Insulin-like growth factor-1 down-regulates the phosphorylation of FXYD1 and rescues behavioral deficits in a mouse model of Rett syndrome. Frontiers in Neuroscience, 14, 20.

Moosavi, S. M., van Reyk, D., Di Bartolo, B., Tang, O., Bubb, K. J., & Figtree, G. A. (2020). Absence of Fxyd1 is Associated With a Female-specific Pro-inflammatory and Hypercholesterolemic Environment: Implications for Atherosclerosis. Circulation, 142(Suppl_3), A16286-A16286.

O’Donnell, A. M., Nakamura, H., Tomuschat, C., Marayati, N. F., & Puri, P. (2019). Abnormal Scn1b and Fxyd1 gene expression in the pulled-through ganglionic colon may influence functional outcome in patients with Hirschsprung’s disease. Pediatric surgery international, 35, 9-14.

Matagne, V., Wondolowski, J., Frerking, M., Shahidullah, M., Delamere, N. A., Sandau, U. S., ... & Ojeda, S. R. (2018). Correcting deregulated Fxyd1 expression rescues deficits in neuronal arborization and potassium homeostasis in MeCP2 deficient male mice. Brain research, 1697, 45-52.

Christiansen, D., Murphy, R. M., Bangsbo, J., Stathis, C. G., & Bishop, D. J. (2018). Increased FXYD1 and PGC‐1α mRNA after blood flow‐restricted running is related to fibre type‐specific AMPK signalling and oxidative stress in human muscle. Acta physiologica, 223(2), e13045.

Bubb, K., Tang, O., Hansen, T., & Figtree, G. (2018). Oxidative modification of the cardiac sodium potassium pump is worsened in the absence of FXYD1, contributing to cardiac dysfunction and fibrosis. Free Radical Biology and Medicine, 128, S21.

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For research use only. Not intended for any clinical use.

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